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    How helminth lipid-binding proteins offload their ligands to membranes: Differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of schistosomes

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    Authors
    McDermott, Lindsay C.
    Kennedy, Malcolm W.
    McManus, Donald P.
    Bradley, Jannette E.
    Cooper, Alan
    Storch, Judith
    Affiliation
    Rutgers University
    University of Glasgow
    Queensland Institute for Medical Research
    University of Nottingham
    Issue Date
    2002-05-28
    Subjects
    n-3 polyunsaturated fatty acids
    C700 Molecular Biology, Biophysics and Biochemistry
    
    Metadata
    Show full item record
    Abstract
    Three different classes of small lipid-binding protein (LBP) are found in helminth parasites. Although of similar size, the ABA-1A1 (also designated As-NPA-A1) and Ov-FAR-1 (formerly known as Ov20) proteins of nematodes are mainly alpha-helical and have no known structural counterparts in mammals, whereas Sj-FABPc of schistosomes is predicted to form a beta-barrel structure similar to the mammalian family of intracellular fatty acid binding proteins. The parasites that produce these proteins are unable to synthesize their own complex lipids and, instead, rely entirely upon their hosts for supply. As a first step in elucidating whether these helminth proteins are involved in the acquisition of host lipid, the process by which these LBPs deliver their ligands to acceptor membranes was examined, by comparing the rates and mechanisms of ligand transfer from the proteins to artificial phospholipid vesicles using a fluorescence resonance energy transfer assay. All three proteins bound the fluorescent fatty acid 2-(9-anthroyloxy)palmitic acid (2AP) similarly, but there were clear differences in the rates and mechanisms of fatty acid transfer. Sj-FABPc displayed a collisional mechanism; 2AP transfer rates increased with acceptor membrane concentration, were modulated by acceptor membrane charge, and were not diminished in the presence of increasing salt concentrations. In contrast, transfer of ligand from Ov-FAR-1 and ABA-1A1 involved an aqueous diffusion step; transfer rates from these proteins were not modulated by acceptor membrane concentration or charge but did decrease with the ionic strength of the buffer. Despite these differences, all of the proteins interacted directly with membranes, as determined using a cytochrome c competition assay, although Sj-FABPc interacted to a greater extent than did Ov-FAR-1 or rABA-1A1. Together, these results suggest that Sj-FABPc is most likely to be involved in the intracellular targeted transport and metabolism of fatty acids, whereas Ov-FAR-1 and ABA-1A1 may behave in a manner analogous to that of extracellular LBPs such as serum albumin and plasma retinol binding protein.
    Citation
    McDermott L, Kennedy MW, McManus DP, Bradley JE, Cooper A, Storch J (2002) 'How helminth lipid-binding proteins offload their ligands to membranes: Differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of schistosomes', Biochemistry, 41 (21), pp.6706-6713.
    Publisher
    American Chemical Society
    Journal
    Biochemistry
    URI
    http://hdl.handle.net/10547/622687
    DOI
    10.1021/bi0159635
    PubMed ID
    12022874
    Additional Links
    https://pubs.acs.org/doi/abs/10.1021/bi0159635
    Type
    Article
    Language
    en
    ISSN
    0006-2960
    ae974a485f413a2113503eed53cd6c53
    10.1021/bi0159635
    Scopus Count
    Collections
    Biomedical and biological science

    entitlement

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