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    Bile UPLC-MS fingerprinting and bile acid fluxes during human liver transplantation

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    Authors
    Legido-Quigley, Cristina
    McDermott, Lindsay C.
    Vilca-Melendez, Hector
    Murphy, Gerard M.
    Heaton, Nigel
    Lindon, John C.
    Nicholson, Jeremy K.
    Holmes, Elaine
    Issue Date
    2011-07-06
    Subjects
    n-3 polyunsaturated fatty acids
    C700 Molecular Biology, Biophysics and Biochemistry
    
    Metadata
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    Abstract
    Bile flow restoration is a crucial step in the recovery process post transplantation of the liver. Here, metabolic trajectories based on changes in bile secretion – a known marker of functionality – have been utilised as an approach for discovering bile fluxes during transplantation. A total of ten liver transplants were monitored and from these 68 bile samples from both donors and recipients were collected and analysed using ultra‐performance LC‐MS in combination with multivariate statistical analysis. Based on the principal component scores constructed from the total bile fingerprint, differentiation of the bile acid concentrations before and after transplantation was detected. A trend was also observed, by constructing metabolic trajectories, whereby the post‐transplant profiles approached the position of pre‐transplant profiles within 30–60 min of the restoration of bile secretion function. The ten major conjugated bile acid salts were measured and a significant increase in concentrations of taurocholic acid and taurochenodeoxycholic acid were seen after transplantation. In addition, the ratios of secondary bile acids detected in gall bladder and hepatic bile were measured before and after transplantation. This study suggests that bile acid ratios in the donor liver at the pre‐transplant and post‐transplant stage may be important and that profiling of secreted bile after transplantation may aid clinical assessment and progress post‐transplantation.
    Citation
    Legido-Quigley C, McDermott L, Vilca-Melendez H, Murphy GM, Heaton N, Lindon JC, Nicholson JK, Holmes E (2011) 'Bile UPLC-MS fingerprinting and bile acid fluxes during human liver transplantation', ELECTROPHORESIS, 32 (15), pp.-.
    Publisher
    Wiley
    Journal
    ELECTROPHORESIS
    URI
    http://hdl.handle.net/10547/622684
    DOI
    10.1002/elps.201000512
    PubMed ID
    21732555
    Additional Links
    https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201000512
    Type
    Article
    Language
    en
    ISSN
    0173-0835
    ae974a485f413a2113503eed53cd6c53
    10.1002/elps.201000512
    Scopus Count
    Collections
    Biomedical and biological science

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