• Login
    View Item 
    •   Home
    • Research from April 2016
    • Biomedical and biological science
    • View Item
    •   Home
    • Research from April 2016
    • Biomedical and biological science
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UOBREPCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartmentThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalDepartment

    My Account

    LoginRegister

    About

    AboutLearning ResourcesResearch Graduate SchoolResearch InstitutesUniversity Website

    Statistics

    Display statistics

    Strong and weak zinc binding sites in human zinc-α2-glycoprotein

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Authors
    Kumar, Aditya Arun
    Hati, Debolina
    Thaker, Thana'a Mohajer
    Miah, Layeque
    Cunningham, Phil
    Domene, Carmen
    Bui, Tam T. T.
    Drake, Alex F.
    McDermott, Lindsay C.
    Affiliation
    King's College London
    Issue Date
    2013-11-01
    Subjects
    zinc
    n-3 polyunsaturated fatty acids
    C700 Molecular Biology, Biophysics and Biochemistry
    
    Metadata
    Show full item record
    Abstract
    Zinc-α2-glycoprotein (ZAG) is an adipokine with an MHC class I-like protein fold. Even though zinc causes ZAG to precipitate from plasma during protein purification, no zinc binding has been identified to date. Using mass spectrometry, we demonstrated that ZAG contains one strongly bound zinc ion, predicted to lie close to the α1 and α2 helical groove. UV, CD and fluorescence spectroscopies detected weak zinc binding to holo-ZAG, which can bind up to 15 zinc ions. Zinc binding to 11-(dansylamino) undecanoic acid was enhanced by holo-ZAG. Zinc binding may be important for ZAG binding to fatty acids and the β-adrenergic receptor.
    Citation
    Kumar, Hati, Thaker, Miah, Cunningham, Domene, Bui, Drake, McDermott (2013) 'Strong and weak zinc binding sites in human zinc-α2-glycoprotein', FEBS Letters, 587 (24), pp.3949-54.
    Publisher
    Wiley
    Journal
    FEBS Letters
    URI
    http://hdl.handle.net/10547/622676
    DOI
    10.1016/j.febslet.2013.10.026
    PubMed ID
    24188824
    Additional Links
    https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2013.10.026
    Type
    Article
    Language
    en
    ISSN
    0014-5793
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.febslet.2013.10.026
    Scopus Count
    Collections
    Biomedical and biological science

    entitlement

    Related articles

    • Zinc-induced oligomerization of zinc α2 glycoprotein reveals multiple fatty acid-binding sites.
    • Authors: Zahid H, Miah L, Lau AM, Brochard L, Hati D, Bui TT, Drake AF, Gor J, Perkins SJ, McDermott LC
    • Issue date: 2016 Jan 1
    • Crystal structure of zinc-α2-glycoprotein in complex with a fatty acid reveals multiple different modes of protein-lipid binding.
    • Authors: Lau AM, Zahid H, Gor J, Perkins SJ, Coker AR, McDermott LC
    • Issue date: 2019 Oct 15
    • Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein.
    • Authors: Delker SL, West AP Jr, McDermott L, Kennedy MW, Bjorkman PJ
    • Issue date: 2004 Nov
    • Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma.
    • Authors: Hassan MI, Bilgrami S, Kumar V, Singh N, Yadav S, Kaur P, Singh TP
    • Issue date: 2008 Dec 19
    • Hydrophobic ligand binding by Zn-alpha 2-glycoprotein, a soluble fat-depleting factor related to major histocompatibility complex proteins.
    • Authors: Kennedy MW, Heikema AP, Cooper A, Bjorkman PJ, Sanchez LM
    • Issue date: 2001 Sep 14
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.