Proteomic analysis of age-related changes in ovine cerebrospinal fluid
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Affiliation
King’s College LondonChang Gung University
University of Bedfordshire
RJAH Orthopaedic Hospital
Keele University
Issue Date
2018-04-21Subjects
proteomic analysisneurodegenerative disease
ageing
cerebrospinal fluid
C700 Molecular Biology, Biophysics and Biochemistry
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Cerebrospinal fluid (CSF) circulates through the brain and has a unique composition reflecting the biological processes of the brain. Identifying ageing CSF biomarkers can aid in understanding the ageing process and interpreting CSF protein changes in neurodegenerative diseases. In this study, ovine CSF proteins from young (1-2 year old), middle aged (3-6 year old) and old (7-10 year old) sheep were systemically studied. CSF proteins were labelled with iTRAQ tagging reagents and fractionated by 2-dimensional high performance, liquid chromatography. Tryptic peptides were identified using MS/MS fragmentation ions for sequencing and quantified from iTRAQ reporter ion intensities at m/z 114, 115, 116 and 117. Two hundred thirty one peptides were detected, from which 143 proteins were identified. There were 52 proteins with >25% increase in concentrations in the old sheep compared to the young. 33 of them increased >25% but <50%, 13 increased >50% but <1 fold, 6 increased >1 fold [i.e. haptoglobin (Hp), haemoglobin, neuroendocrine protein 7B2, IgM, fibrous sheath interacting protein 1, vimentin]. There were 18 proteins with >25% decrease in concentrations in the old sheep compared to the young. 17 of them decreased >25% but <50%, and histone deacetylase 7 (HDAC7) was gradually decreased for over 80%. Glutathione S-transferase was decreased in middle aged CSF compared to both young and old CSF. The differential expressions of 3 proteins (Hp, neuroendocrine protein 7B2, IgM) were confirmed by immunoassays. These data expand our current knowledge regarding ovine CSF proteins, supply the necessary information to understand the ageing process in the brain and provide a basis for diagnosis of neurodegenerative diseases.Citation
Chen C, Preston JE,Zhou S, Fuller HR, Morgan D, Chen R (2018) 'Proteomic analysis of age-related changes in ovine cerebrospinal fluid', Experimental Gerontology, 108, pp.181-188.Publisher
ElsevierJournal
Experimental GerontologyPubMed ID
29704639Additional Links
https://www.sciencedirect.com/science/article/pii/S0531556518301633Type
ArticleLanguage
enISSN
0531-5565Sponsors
the Biotechnology and Biological Sciences Research Council (BBSRC) (BBD01381X1), the Wellcome Trust (200633/z/16/z), the Physiology Society (summer vacation studentship)ae974a485f413a2113503eed53cd6c53
10.1016/j.exger.2018.04.012
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