Regulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsia
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Issue Date
2014-09-25Subjects
n-3 polyunsaturated fatty acidspre-eclampsia
fatty acid binding proteins
hypoxia
long chain polunsatured fatty acids
hypoxia-inducible factor-1 alpha
placenta
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Pre-eclampsia is characterized by placental hypoxia and dyslipidemia. Arachidonic and docosahexanoic acids are essential maternal nutrients for fetal development. They are transported via placental trophoblast cells by membrane and cytosolic fatty acid binding proteins. Others report the expressions of these proteins which are increased in hypoxic trophoblasts. Using bioinformatics, BeWo cells, reporter assays, quantitative real-time PCR and immunoblotting we tested the hypothesis that hypoxia inducible factors 1α (HIF-1α) and/ or 2α (HIF-2α) regulate the expressions of FABP1, FABP3, FABP4 and FATP2 proteins. Three hypoxia responsive elements (HRE) were identified in FABP1 which cumulatively responded strongly to HIF-1α and weakly to HIF-2α. FABP3 expression partially responded to HIF-1α. Two putative HRE were validated in FABP4 both of which responded weakly toHIF-1α and HIF-2α. FATP2 protein expression reacted positively to hypoxia. Thus, fetal essential fatty acid supply via the placenta is protected under hypoxia. It will be interesting to determine if our findings are replicated in human pre-eclamptic placenta.Citation
Jadoon A, Cunningham P, McDermott LC (2014) 'Regulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsia', Prostaglandins, Leukotrienes and Essential Fatty Acids, 93, pp.25-29.Publisher
ElsevierAdditional Links
http://www.plefa.com/article/S0952-3278(14)00148-3/fulltextType
ArticleLanguage
enISSN
0952-3278Sponsors
EPSRC DTA award EP/P504961/1ae974a485f413a2113503eed53cd6c53
10.1016/j.plefa.2014.09.004