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dc.contributor.authorWang, QingBiaoen
dc.contributor.authorXu, Yiqinen
dc.contributor.authorGu, Zhuoyaen
dc.contributor.authorLiu, Nianen
dc.contributor.authorJin, Keen
dc.contributor.authorLi, Yaoen
dc.contributor.authorCrabbe, M. James C.en
dc.contributor.authorZhong, Yangen
dc.date.accessioned2018-01-19T14:26:44Z
dc.date.available2018-01-19T14:26:44Z
dc.date.issued2017-11-21
dc.identifier.citationWang Q.B., Xu Y., Gu Z., Liu N., Jin K., Li Y., Crabbe,M.J.C., Zhong Y. (2018) 'Identification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sites', Computational Biology and Chemistry 73 25-30en
dc.identifier.issn1476-9271
dc.identifier.pmid29413813
dc.identifier.doi10.1016/j.compbiolchem.2017.11.002
dc.identifier.urihttp://hdl.handle.net/10547/622465
dc.description.abstractBacterial RNA polymerase (RNAP) is an effective target for antibacterial treatment. In order to search new potential targets in RNAP of Mycobacterium, we detected adaptive selections of RNAP related genes in 13 strains of Mycobacterium by phylogenetic analysis. We first collected sequences of 17 genes including rpoA, rpoB, rpoC, rpoZ, and sigma factor A-M. Then maximum likelihood trees were constructed, followed by positive selection detection. We found that sigG shows positive selection along the clade (M. tuberculosis, M. bovis), suggesting its important evolutionary role and its potential to be a new antibacterial target. Moreover, the regions near 933Cys and 935His on the rpoB subunit of M. tuberculosis showed significant positive selection, which could also be a new attractive target for anti-tuberculosis drugs.
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1476927116303164en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectphylogenetic analysisen
dc.subjectselective pressureen
dc.subjectMycobacterium tuberculosisen
dc.titleIdentification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sitesen
dc.typeArticleen
dc.identifier.eissn1476-9271
dc.contributor.departmentFudan Universityen
dc.contributor.departmentOxford Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.contributor.departmentTibet Universityen
dc.identifier.journalComputational Biology and Chemistryen
dc.date.updated2018-01-19T14:22:53Z
refterms.dateFOA2018-11-21T00:00:00Z
html.description.abstractBacterial RNA polymerase (RNAP) is an effective target for antibacterial treatment. In order to search new potential targets in RNAP of Mycobacterium, we detected adaptive selections of RNAP related genes in 13 strains of Mycobacterium by phylogenetic analysis. We first collected sequences of 17 genes including rpoA, rpoB, rpoC, rpoZ, and sigma factor A-M. Then maximum likelihood trees were constructed, followed by positive selection detection. We found that sigG shows positive selection along the clade (M. tuberculosis, M. bovis), suggesting its important evolutionary role and its potential to be a new antibacterial target. Moreover, the regions near 933Cys and 935His on the rpoB subunit of M. tuberculosis showed significant positive selection, which could also be a new attractive target for anti-tuberculosis drugs.


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