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dc.contributor.authorXu, Weilien
dc.contributor.authorHe, Panen
dc.contributor.authorHe, Shenghuaen
dc.contributor.authorCui, Pengjuen
dc.contributor.authorMi, Yaqingen
dc.contributor.authorYang, Yangen
dc.contributor.authorLi, Yangen
dc.contributor.authorZhou, Shaoboen
dc.date.accessioned2018-01-16T09:47:30Z
dc.date.available2018-01-16T09:47:30Z
dc.date.issued2018-01-15
dc.identifier.citationXu W, He P, He, Cui P, Mi Y, Yang Y, Li Y, Zhou S. (2018) 'Gamma-tocotrienol stimulates the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells', Journal of Chemistry, 2018.en
dc.identifier.issn2090-9063
dc.identifier.doi10.1155/2018/3805932
dc.identifier.urihttp://hdl.handle.net/10547/622463
dc.description.abstractGamma-tocotrienol, a major component of tocotrienol-rich fraction of palm oil, has been suggested to exhibit bone protective effects in vivo. However, the effects of γ-tocotrienol on osteoblast cells are still unclear. In this study, the effects of γ-tocotrienol on the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells were investigated. Our results showed that γ-tocotrienol (2–8 μmol/L) significantly improved the cell proliferation (), but it did not affect cell cycle progression. γ-Tocotrienol significantly increased alkaline phosphatase (ALP) activity (), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells. Meanwhile, we found that γ-tocotrienol is promoted in differentiation MC3T3-E1 cells by upregulation of the expression of Runx2 protein. Moreover, the number of bone nodules increased over 2.5-fold in cells treated with γ-tocotrienol (2–8 μmol/L) for 24 d compared to control group. These results indicated that γ-tocotrienol at low dose levels, especially 4 μmol/L, could markedly enhance the osteoblastic function by increasing the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells. Moreover, our data also indicated that Runx2 protein may be involved in these effects. Further studies are needed to determine the potential of γ-tocotrienol as an antiosteoporotic agent.
dc.language.isoenen
dc.publisherHindawi Publishing Corporationen
dc.relation.urlhttps://www.hindawi.com/journals/jchem/2018/3805932/en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectosteoporosisen
dc.subjectboneen
dc.subjectgamma-tocotrienolen
dc.titleGamma-tocotrienol stimulates the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cellsen
dc.typeArticleen
dc.identifier.eissn2090-9071
dc.contributor.departmentHarbin Institute of Technologyen
dc.contributor.departmentHarbin Medical Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalJournal of Chemistryen
dc.date.updated2018-01-16T09:42:41Z
dc.description.noteopen access
html.description.abstractGamma-tocotrienol, a major component of tocotrienol-rich fraction of palm oil, has been suggested to exhibit bone protective effects in vivo. However, the effects of γ-tocotrienol on osteoblast cells are still unclear. In this study, the effects of γ-tocotrienol on the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells were investigated. Our results showed that γ-tocotrienol (2–8 μmol/L) significantly improved the cell proliferation (), but it did not affect cell cycle progression. γ-Tocotrienol significantly increased alkaline phosphatase (ALP) activity (), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells. Meanwhile, we found that γ-tocotrienol is promoted in differentiation MC3T3-E1 cells by upregulation of the expression of Runx2 protein. Moreover, the number of bone nodules increased over 2.5-fold in cells treated with γ-tocotrienol (2–8 μmol/L) for 24 d compared to control group. These results indicated that γ-tocotrienol at low dose levels, especially 4 μmol/L, could markedly enhance the osteoblastic function by increasing the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells. Moreover, our data also indicated that Runx2 protein may be involved in these effects. Further studies are needed to determine the potential of γ-tocotrienol as an antiosteoporotic agent.


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