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    Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum

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    Authors
    Chen, Haikui
    Feng, Yang
    Wang, Lina
    Yonezawa, Takahiro
    Crabbe, M. James C.
    Zhang, Xiu
    Zhong, Yang
    Affiliation
    Beifang University of Nationalities, Yinchuan
    Fudan University, Shanghai
    University of Oxford
    University of Bedfordshire
    Tibet University
    Issue Date
    2015-02-15
    Subjects
    environment
    Lycium ruthenicum
    UV-B
    D447 Environmental Conservation
    
    Metadata
    Show full item record
    Abstract
    Ultraviolet-B (UV-B) is a natural component of the solar radiation. Due to its high energy, low dosages of UV-B can bring huge potential damage effect to organisms. Despite much research that has analyzed the gene expression changes of plants that under UV-B radiation, the transcriptome response of Lycium ruthenicum under the UV-B induction is still unavailable. The aim of our study was to identify UV-B responsive genes and gain an insight into the underlying genetic basis of the pathobiology of UV-B related damage. We collected leaf samples from L. ruthenicum with and without UV-B exposure, and then performed a transcriptome profiling to comprehensively investigate their expression signatures. By employing the high throughput RNA-sequencing analysis of samples with and without UV-B exposure, we identified 1,913 up-regulated and 536 down-regulated genes at least by twofold changes. The activity of antioxidant enzyme related genes, including the superoxide dismutase, was decreased, genes related to the synthesis of secondary metabolites and defense responses, such as cinnamyl alcohol dehydrogenase, chalcone-flavanone isomerase and dihydroflavonol reductase were also downregulated. The expression patterns of 14 randomly selected genes resulted from quantitative real-time PCR were basically consistent with their transcript abundance changes identified by RNA-sequencing. We found that several biological pathways related to biotic and abiotic stresses, including cell defense, photosynthesis processes, energy metabolism, were involved in the process of UV-B stress response. A genome-wide screening of gene deregulation under UV-B induction would provide an insight into the understanding of the molecular bases and pathogenesis of UV-B responses.
    Citation
    Chen H., Feng Y., Wang L., Yonezawa T., Crabbe M.J.C., Zhang X., Zhong Y. (2015) 'Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum', Molecular Biology Reports, 42 (3), pp.639-649.
    Publisher
    Springer
    Journal
    Molecular Biology Reports
    URI
    http://hdl.handle.net/10547/622283
    DOI
    10.1007/s11033-014-3809-y
    PubMed ID
    25366177
    Additional Links
    https://link.springer.com/article/10.1007/s11033-014-3809-y
    Type
    Article
    Language
    en
    ISSN
    0301-4851
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11033-014-3809-y
    Scopus Count
    Collections
    Biomedical and biological science

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