• A dynamic feedback algorithm of AFM based on cell morphology changes

      Cheng, Can; Wang, Xinyue; Dong, Jianjun; Liu, Zimin; Wang, Zuobin; Changchun University of Science and Technology; University of Bedfordshire (IEEE, 2021-11-18)
      The atomic force microscope (AFM) used in biological research as a powerful tool has been for many years. However, the imaging of living cells is still a problem as the sample is too high and too soft to obtain their accurate morphologies. Especially in the high-speed scanning mode, AFM does not have enough judgments to make accurate measurements at the down part of the sample. In this study, we propose an improved control method to improve the image quality of living human colon cancer cells (SW480) especially in the down part during the scanning.
    • Effect of astragalus polysaccharides on cancer cells studied by AFM

      Lu, Zhengcheng; Wang, Zuobin; Li, Dayou; Zhu, Wenyu; Wang, Rui; Qu, Kaige; Yan, Jin; University of Bedfordshire; Changchun University of Science and Technology (IEEE, 2021-11-18)
      As a traditional Chinese medicine, astragalus and its products are used in cancer treatment aiming to reduce the side effects of chemotherapy. Cells are the most basic unit of living organisms, and AFM directly obtains information from living cells on the micro/nano scale. Therefore, the use of AFM to study the interaction between astragalus and cells is conducive to a full range of drug efficacy evaluation and provides a new way for drug development. In this paper, astragalus polysaccharides were extracted from astragalus, which were diluted into solutions of different concentrations. Combined with the MTT experiment, the effects of Astragalus polysaccharide on cancer cells and benign cells were studied by AFM.