• Cell spreading behaviors on hybrid nanopillar and nanohole arrays

      Wu, Xiaomin; Li, Li; Wang, Lu; Lei, Zecheng; Yang, Fan; Liu, Ri; Wang, Ying; Peng, Kuiqing; Wang, Zuobin; Wu X (IOP Publishing, 2021-11-05)
      Although nanopillars (NPs) provide a promising tool for capturing tumor cells, the effect of mixing NPs with other nanopatterns on cell behavior remains to be further studied. In this paper, a method of fabricating silicon nanoscale topographies by combining laser interference lithography with metal assisted chemical etching was introduced to investigate the behaviors and pseudopodia of A549 cells on the topologies. It was found that cells had a limited manner in spreading with small cell areas on the silicon nanopillar (SiNP) arrays, but a good manner in spreading with large cell areas on the silicon nanohole (SiNH) arrays. When on the hybrid SiNP/SiNH arrays, cells had medium cell areas and they arranged orderly along the boundaries of SiNPs and SiNHs, as well as 80% of cells displayed a preference for SiNPs over SiNHs. Furthermore, the lamellipodia and filopodia are dominant in the hybrid SiNP/SiNH and SiNP arrays, respectively, both of them are dominant in the SiNH arrays. In addition, the atomic force acoustic microscopy was also employed to detect the subsurface features of samples. The results suggest that the hybrid SiNP/SiNH arrays have a targeted trap and elongation effect on cells. The findings provide a promising method in designing hybrid nanostructures for efficient tumor cell traps, as well as regulating the cell behaviors and pseudopodia.
    • Electrical conductivity measurement of λ DNA molecules by conductive atomic force microscopy

      Wang, Ying; Xie, Ying; Gao, Mingyan; Zhang, Wenxiao; Liu, Lanjiao; Qu, Yingmin; Wang, Jiajia; Hu, Cuihua; Song, Zhengxun; Wang, Zuobin; et al. (IOP Publishing, 2021-11-08)
      Conductive atomic force microscopy (C-AFM) is a powerful tool used in the microelectronics analysis by applying a certain bias voltage between the conducting probe and the sample and obtaining the electrical information of sample. In this work, the surface morphological information and current images of the lambda DNA (λ DNA) molecules with different distributions were obtained by C-AFM. The 1 and 10 ng μl−1 DNA solutions were dripped onto mica sheets for making randomly distributed DNA and DNA network samples, and another 1 ng μl−1 DNA sample was placed in a DC electric field with a voltage of 2 V before being dried for stretching the DNA sample. The results show that the current flowing through DNA networks was significantly higher than the stretched and random distribution of DNA in the experiment. The I–V curve of DNA networks was obtained by changing the bias voltage of C-AFM from −9 to 9 V. The currents flowing through stretched DNA at different pH values were studied. When the pH was 7, the current was the smallest, and the current was gradually increased as the solution became acidic or alkaline.
    • Self-assembly of DNA molecules in magnetic fields

      Gao, Mingyan; Hu, Jing; Wang, Jianfei; Liu, Mengnan; Zhu, Xiaona; Saeed, Sadaf; Hu, Cuihua; Song, Zhengxun; Xu, Hongmei; Wang, Zuobin; et al. (IOP Publishing, 2021-11-18)
      In this work, a rich variety of self-assembled DNA patterns were obtained in the magnetic field. Herein, atomic force microscopy (AFM) was utilized to investigate the effects of the concentration of DNA solution, intensity and direction of magnetic field and modification of mica surface by different cations on the self-assembly of DNA molecules. It was found that owning to the change of the DNA concentration, even under the same magnetic field, the DNA self-assembly results were different. The in situ test results showed that the DNA self-assembly in an magnetic field was more likely to occur in liquid phase than in gas phase. In addition, whether in a horizontal or vertical magnetic field, a single stretched dsDNA was obtained in a certain DNA concentration and magnetic field intensity. Besides, the modification of cations on the mica surface significantly increased the force between the DNA molecules and mica surface, and further changed the self-assembly of DNA molecules under the action of magnetic field.