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    Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes

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    Authors
    Tsai, Sujune
    Chen, Jiann-Chu
    Spikings, Emma
    Li, Jan-Jung
    Lin, Chiahsin
    Affiliation
    Mingdao University
    National Taiwan Ocean University
    University of Bedfordshire
    Nationall Museum Marine Biology & Aquarium, Taiwan
    National Dong Hwa University
    Issue Date
    2015-06
    
    Metadata
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    Abstract
    A critical step for successful cryopreservation is to determine the optimal cryoprotectant treatment that can provide protective effects against cryoinjury during freezing and with minimal toxicity. Most cryoprotectants have chemical and osmotic effects when used at high concentrations. Cryoprotectants can damage coral mitochondrial distributions and membrane potentials, which results in reduced ATP production. As mitochondrial DNA (mtDNA) encodes for components of the electron transport chain (ETC) and plays a critical role in ATP synthesis capacity, we determined the effects of cryoprotectants on mtDNA in hard coral (Echinopora spp.) oocytes using quantitative real-time PCR. Our results showed that an insult from a cryoprotectant may be compensated for by the genetic defense mechanisms of these cells. Methanol was found to have the least effect on coral oocytes with regard to their energy status. A single oocyte without cryoprotectant treatment produced an average of 4,220,645 ± 169,990 mtDNA copies, which was greater than that in mammals. However, relatively lower mtDNA copy numbers (<2,000,000) were observed when oocytes were treated with dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), or glycerol at a concentration of 3 M for 20 min. These results provide direct evidence that hard coral (Echinopora spp.) oocytes are extremely susceptible to cryoprotectants and support the concerns with regard to the adverse effects of cryoprotectants.
    Citation
    Tsai, S.J., Chen, J.C., Spikings, E., Li, J.J. and Lin, C.H. (2015) 'Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes', Mitochondrial DNA, 26(3), pp.420-425.
    Publisher
    Informa Healthcare
    Journal
    Mitochondrial DNA
    URI
    http://hdl.handle.net/10547/560930
    DOI
    10.3109/19401736.2013.855734
    Additional Links
    http://informahealthcare.com/doi/abs/10.3109/19401736.2013.855734
    Type
    Article
    Language
    en
    ISSN
    1940-1736
    1940-1744
    ae974a485f413a2113503eed53cd6c53
    10.3109/19401736.2013.855734
    Scopus Count
    Collections
    Biomedical Science

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