Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes
Affiliation
Mingdao UniversityNational Taiwan Ocean University
University of Bedfordshire
Nationall Museum Marine Biology & Aquarium, Taiwan
National Dong Hwa University
Issue Date
2015-06
Metadata
Show full item recordAbstract
A critical step for successful cryopreservation is to determine the optimal cryoprotectant treatment that can provide protective effects against cryoinjury during freezing and with minimal toxicity. Most cryoprotectants have chemical and osmotic effects when used at high concentrations. Cryoprotectants can damage coral mitochondrial distributions and membrane potentials, which results in reduced ATP production. As mitochondrial DNA (mtDNA) encodes for components of the electron transport chain (ETC) and plays a critical role in ATP synthesis capacity, we determined the effects of cryoprotectants on mtDNA in hard coral (Echinopora spp.) oocytes using quantitative real-time PCR. Our results showed that an insult from a cryoprotectant may be compensated for by the genetic defense mechanisms of these cells. Methanol was found to have the least effect on coral oocytes with regard to their energy status. A single oocyte without cryoprotectant treatment produced an average of 4,220,645 ± 169,990 mtDNA copies, which was greater than that in mammals. However, relatively lower mtDNA copy numbers (<2,000,000) were observed when oocytes were treated with dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), or glycerol at a concentration of 3 M for 20 min. These results provide direct evidence that hard coral (Echinopora spp.) oocytes are extremely susceptible to cryoprotectants and support the concerns with regard to the adverse effects of cryoprotectants.Citation
Tsai, S.J., Chen, J.C., Spikings, E., Li, J.J. and Lin, C.H. (2015) 'Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes', Mitochondrial DNA, 26(3), pp.420-425.Publisher
Informa HealthcareJournal
Mitochondrial DNAAdditional Links
http://informahealthcare.com/doi/abs/10.3109/19401736.2013.855734Type
ArticleLanguage
enISSN
1940-17361940-1744
ae974a485f413a2113503eed53cd6c53
10.3109/19401736.2013.855734