Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
Authors
Eastwood, Daniel C.Herman, Bram
Noble, Ralph
Dobrovin-Pennington, Andreja
Sreenivasaprasad, Surapareddy
Burton, Kerry S.
Affiliation
University of SwanseaUniversity of Warwick
East Malling Research
University of Bedfordshire
Agilent Technologies UK Ltd.
Issue Date
2013
Metadata
Show full item recordAbstract
Reproductive phase change from vegetative mycelium to the initiation of fruiting in Agaricus bisporus is regulated in large part by the sensing of environmental conditions. A model is proposed in which three separate environmental factors exert control at different stages of the reproductive developmental process change. The eight carbon volatile 1-octen-3-ol controls the early differentiation from vegetative hyphae to multicellular knots; temperature reduction is essential for the later differentiation of primodia; and carbon dioxide level exerts quantitative control on the number of fruiting bodies developed. Analysis of transcriptomic changes during the reproductive phase change was carried out with initiation-specific microarrays, and the newly published A. bisporus genome was used to analyse the promoter regions of differentially regulated genes. Our studies have shown there to be both early and late initiation responses relating to sensing of eight carbon volatiles and temperature respectively. A subset of 45 genes was transcriptionally regulated during the reproductive phase change which exhibited a range of functions including cell structure, nitrogen and carbon metabolism, and sensing and signalling. Three gene clusters linking increased transcription with developmental stage were identified. Analysis of promoter regions revealed cluster-specific conserved motifs indicative of co-ordinated regulation of transcriptionCitation
Eastwood, D.C., Herman, B., Noble, R., Dobrovin-Pennington, A., Sreenivasaprasad, S. and Burton, K.S. (2013) 'Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2', Fungal Genetics and Biology, 55,pp.54-66Publisher
ElsevierJournal
Fungal Genetics and BiologyAdditional Links
http://linkinghub.elsevier.com/retrieve/pii/S1087184513000030Type
ArticleLanguage
enISSN
10871845ae974a485f413a2113503eed53cd6c53
10.1016/j.fgb.2013.01.001