Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors
AuthorsAhmed, Bushra Y.
Hermens, Wim TJMC
Niclou, Simone P.
Anderson, Patrick N.
AffiliationUniversity College London
Netherlands Institute for Brain Research
Netherlands Ophthalmic Research Institute
University of Nottingham
MetadataShow full item record
AbstractInactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain
CitationAhmed B.Y., Chakravarthy S., Eggers R., et al. (2004) 'Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors', BMC Neuroscience, 5(1):4-13
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