Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors
Authors
Ahmed, Bushra Y.Chakravarthy, Sridhara
Eggers, Ruben
Hermens, Wim TJMC
Zhang, Jing
Niclou, Simone P.
Levelt, Christiaan
Sablitzky, Fred
Anderson, Patrick N.
Lieberman, A.R.
Verhaagen, Joost
Affiliation
University College LondonNetherlands Institute for Brain Research
Netherlands Ophthalmic Research Institute
University of Nottingham
Issue Date
2004
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Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrainCitation
Ahmed B.Y., Chakravarthy S., Eggers R., et al. (2004) 'Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors', BMC Neuroscience, 5(1):4-13Publisher
BioMed CentralJournal
BMC NeuroscienceAdditional Links
http://www.biomedcentral.com/1471-2202/5/4Type
ArticleLanguage
enISSN
1471-2202ae974a485f413a2113503eed53cd6c53
10.1186/1471-2202-5-4
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