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dc.contributor.authorInglis, Neil F.en_GB
dc.contributor.authorStevenson, Karenen_GB
dc.contributor.authorHosie, Arthur H.F.en_GB
dc.contributor.authorSharp, J.Michaelen_GB
dc.date.accessioned2013-06-14T12:16:36Z
dc.date.available2013-06-14T12:16:36Z
dc.date.issued1994
dc.identifier.citationInglis, N.F., Stevenson, K., Hosie, A.H.F. and Sharp, J.M. (1994) 'Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum', Gene, 150(1), pp.205-206en_GB
dc.identifier.issn0378-1119
dc.identifier.doi10.1016/0378-1119(94)90889-3
dc.identifier.urihttp://hdl.handle.net/10547/293972
dc.description.abstractA gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/0378111994908893en_GB
dc.rightsArchived with thanks to Geneen_GB
dc.subjectbacterioferritinen_GB
dc.subjectnucleotide sequenceen_GB
dc.subjectexpressionen_GB
dc.subjectMycobacterium aviumen_GB
dc.subjectMycobacterium lepraeen_GB
dc.titleComplete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticumen
dc.typeArticleen
dc.identifier.journalGeneen_GB
html.description.abstractA gene encoding the bacterioferritin subunit (Bfr) of Mycobacterium avium (Ma) subspecies silvaticum has been cloned, sequenced and expressed. The 477-bp open reading frame codes for 159 amino acids, which were shown to share up to 92% identity with the Bfr of five bacterial genera. The recombinant Bfr exhibits serological cross-reactivity with Ma paratuberculosis antigen D, a protein of approx. 20 kDa in cell lysates of Ma paratuberculosis and Ma silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.


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