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    A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals

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    Authors
    Crabbe, M. James C.
    Issue Date
    2003
    Subjects
    FTA cards
    DNA
    rRNA
    Zooxanthellae
    Cnidaria
    
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    Abstract
    We have developed a new simple method for transport, storage, and analysis of genetic material from the corals Agaricia agaricites, Dendrogyra cylindrica, Eusmilia ancora, Meandrina meandrites, Montastrea annularis, Porites astreoides, Porites furcata, Porites porites, and Siderastrea siderea at room temperature. All species yielded sufficient DNA from a single FTA® card (19 μg–43 ng) for subsequent PCR amplification of both coral and zooxanthellar DNA. The D1 and D2 variable region of the large subunit rRNA gene (LSUrDNA) was amplified from the DNA of P. furcata and S. siderea by PCR. Electrophoresis yielded two major DNA bands: an 800-base pair (bp) DNA, which represented the coral ribosomal RNA (rRNA) gene, and a 600-bp DNA, which represented the zooxanthellar srRNA gene. Extraction of DNA from the bands yielded between 290 μg total DNA (S. siderea coral DNA) and 9 μg total DNA (P. furcata zooxanthellar DNA). The ability to transport and store genetic material from scleractinian corals without resort to laboratory facilities in the field allows for the molecular study of a far wider range and variety of coral sites than have been studied to date.
    Citation
    Crabbe, M.C. (2003) 'A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals', Journal of Biochemical and Biophysical Methods, 57 (2),pp.171-176
    Publisher
    Elsevier
    Journal
    Journal of Biochemical and Biophysical Methods
    URI
    http://hdl.handle.net/10547/293946
    DOI
    10.1016/S0165-022X(03)00051-4
    Additional Links
    http://www.ncbi.nlm.nih.gov/pubmed/12915008
    Type
    Article
    Language
    en
    ISSN
    0165-022X
    ae974a485f413a2113503eed53cd6c53
    10.1016/S0165-022X(03)00051-4
    Scopus Count
    Collections
    Environmental Monitoring Research Group

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