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Mapping the Sinorhizobium Meliloti 1021 solute-binding protein-dependent transportome

dc.contributor.authorMauchline, T.H.en_GB
dc.contributor.authorFowler, J.E.en_GB
dc.contributor.authorEast, A.K.en_GB
dc.contributor.authorSartor, A.L.en_GB
dc.contributor.authorZaheer, R.en_GB
dc.contributor.authorHosie, Arthur H.F.en_GB
dc.contributor.authorPoole, Philip S.en_GB
dc.contributor.authorFinan, T.M.en_GB
dc.date.accessioned2013-06-14T10:18:02Z
dc.date.available2013-06-14T10:18:02Z
dc.date.issued2006
dc.identifier.citationMauchline, T.H.,Fowler, J.E., East, A.K., Sartor, A.L.,Zaheer, R., Hosie, A.H.F., Poole, P.S. and Finan, T.M. (2006) 'Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome', Proceedings of the National Academy of Sciences, 103 (47), pp.17933-17938en_GB
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.doi10.1073/pnas.0606673103
dc.identifier.urihttp://hdl.handle.net/10547/293937
dc.description.abstractThe number of solute-binding protein-dependent transporters in rhizobia is dramatically increased compared with the majority of other bacteria so far sequenced. This increase may be due to the high affinity of solute-binding proteins for solutes, permitting the acquisition of a broad range of growth-limiting nutrients from soil and the rhizosphere. The transcriptional induction of these transporters was studied by creating a suite of plasmid and integrated fusions to nearly all ATP-binding cassette (ABC) and tripartite ATP-independent periplasmic (TRAP) transporters of Sinorhizobium meliloti. In total, specific inducers were identified for 76 transport systems, amounting to ≈47% of the ABC uptake systems and 53% of the TRAP transporters in S. meliloti. Of these transport systems, 64 are previously uncharacterized in Rhizobia and 24 were induced by solutes not known to be transported by ABC- or TRAP-uptake systems in any organism. This study provides a global expression map of one of the largest transporter families (transportome) and an invaluable tool to both understand their solute specificity and the relationships between members of large paralogous families
dc.language.isoenen
dc.publisherNational Academy of Sciencesen_GB
dc.relation.urlhttp://www.pnas.org/cgi/doi/10.1073/pnas.0606673103en_GB
dc.rightsArchived with thanks to Proceedings of the National Academy of Sciencesen_GB
dc.subjectATP-binding cassetteen_GB
dc.subjectexpressionen_GB
dc.subjecttripartite ATP-independent periplasmicen_GB
dc.titleMapping the Sinorhizobium Meliloti 1021 solute-binding protein-dependent transportomeen
dc.typeArticleen
dc.identifier.journalProceedings of the National Academy of Sciencesen_GB
html.description.abstractThe number of solute-binding protein-dependent transporters in rhizobia is dramatically increased compared with the majority of other bacteria so far sequenced. This increase may be due to the high affinity of solute-binding proteins for solutes, permitting the acquisition of a broad range of growth-limiting nutrients from soil and the rhizosphere. The transcriptional induction of these transporters was studied by creating a suite of plasmid and integrated fusions to nearly all ATP-binding cassette (ABC) and tripartite ATP-independent periplasmic (TRAP) transporters of Sinorhizobium meliloti. In total, specific inducers were identified for 76 transport systems, amounting to ≈47% of the ABC uptake systems and 53% of the TRAP transporters in S. meliloti. Of these transport systems, 64 are previously uncharacterized in Rhizobia and 24 were induced by solutes not known to be transported by ABC- or TRAP-uptake systems in any organism. This study provides a global expression map of one of the largest transporter families (transportome) and an invaluable tool to both understand their solute specificity and the relationships between members of large paralogous families


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