Mapping the Sinorhizobium Meliloti 1021 solute-binding protein-dependent transportome
Authors
Mauchline, T.H.Fowler, J.E.
East, A.K.
Sartor, A.L.
Zaheer, R.
Hosie, Arthur H.F.
Poole, Philip S.
Finan, T.M.
Issue Date
2006
Metadata
Show full item recordAbstract
The number of solute-binding protein-dependent transporters in rhizobia is dramatically increased compared with the majority of other bacteria so far sequenced. This increase may be due to the high affinity of solute-binding proteins for solutes, permitting the acquisition of a broad range of growth-limiting nutrients from soil and the rhizosphere. The transcriptional induction of these transporters was studied by creating a suite of plasmid and integrated fusions to nearly all ATP-binding cassette (ABC) and tripartite ATP-independent periplasmic (TRAP) transporters of Sinorhizobium meliloti. In total, specific inducers were identified for 76 transport systems, amounting to ≈47% of the ABC uptake systems and 53% of the TRAP transporters in S. meliloti. Of these transport systems, 64 are previously uncharacterized in Rhizobia and 24 were induced by solutes not known to be transported by ABC- or TRAP-uptake systems in any organism. This study provides a global expression map of one of the largest transporter families (transportome) and an invaluable tool to both understand their solute specificity and the relationships between members of large paralogous familiesCitation
Mauchline, T.H.,Fowler, J.E., East, A.K., Sartor, A.L.,Zaheer, R., Hosie, A.H.F., Poole, P.S. and Finan, T.M. (2006) 'Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome', Proceedings of the National Academy of Sciences, 103 (47), pp.17933-17938Publisher
National Academy of SciencesAdditional Links
http://www.pnas.org/cgi/doi/10.1073/pnas.0606673103Type
ArticleLanguage
enISSN
0027-84241091-6490
ae974a485f413a2113503eed53cd6c53
10.1073/pnas.0606673103