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dc.contributor.authorBanham, Janet E.en_GB
dc.contributor.authorBaker, Christopher M.en_GB
dc.contributor.authorCeola, Stefanoen_GB
dc.contributor.authorDay, Iain J.en_GB
dc.contributor.authorGrant, Guy H.en_GB
dc.contributor.authorGroenen, Edgar J.J.en_GB
dc.contributor.authorRodgers, Christopher T.en_GB
dc.contributor.authorJeschke, Gunnaren_GB
dc.contributor.authorTimmel, Christiane R.en_GB
dc.date.accessioned2012-06-14T11:51:19Z
dc.date.available2012-06-14T11:51:19Z
dc.date.issued2008-04
dc.identifier.citationBanham, J., Baker, C.M., Ceola, S., Day, I.J., Grant, G.H., Groenen, E.J.J., Rodgers, C.T., Jeschke, G., Timmel, C.R. (2008) 'Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides', Journal of magnetic resonance 191 (2):202-218en_GB
dc.identifier.issn1090-7807
dc.identifier.pmid18280189
dc.identifier.doi10.1016/j.jmr.2007.11.023
dc.identifier.urihttp://hdl.handle.net/10547/228939
dc.description.abstractInter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18280189en_GB
dc.subjectDEERen_GB
dc.subjectEPR spectroscopyen_GB
dc.subjectstructure elucidationen_GB
dc.subjectdipolar interactionsen_GB
dc.subjectspin labelsen_GB
dc.subject.meshAlgorithms
dc.subject.meshComputer Simulation
dc.subject.meshElectron Spin Resonance Spectroscopy
dc.subject.meshModels, Chemical
dc.subject.meshPeptides
dc.subject.meshSpin Labels
dc.titleDistance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.en
dc.typeArticleen
dc.contributor.departmentUniversity of Oxforden_GB
dc.identifier.journalJournal of magnetic resonance (San Diego, Calif. : 1997)en_GB
html.description.abstractInter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.


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