Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.
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Authors
Banham, Janet E.Baker, Christopher M.
Ceola, Stefano
Day, Iain J.
Grant, Guy H.
Groenen, Edgar J.J.
Rodgers, Christopher T.
Jeschke, Gunnar
Timmel, Christiane R.
Affiliation
University of OxfordIssue Date
2008-04
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Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.Citation
Banham, J., Baker, C.M., Ceola, S., Day, I.J., Grant, G.H., Groenen, E.J.J., Rodgers, C.T., Jeschke, G., Timmel, C.R. (2008) 'Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides', Journal of magnetic resonance 191 (2):202-218Publisher
ElsevierPubMed ID
18280189Additional Links
http://www.ncbi.nlm.nih.gov/pubmed/18280189Type
ArticleLanguage
enISSN
1090-7807ae974a485f413a2113503eed53cd6c53
10.1016/j.jmr.2007.11.023
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