Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos
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Abstract
Zebrafish embryos have not been cryopreserved due to their structural limitations. Although embryo survival rates have been used as the measured outcome for most of the cryopreservation protocols studied, there are very limited data available at the molecular level. This study focused on the effect of chilling and subsequent warming on gene expression of sox2, sox3 and sox19a which play vital roles in the development of zebrafish embryos. A quantitative RT-PCR approach was used to investigate gene expression following chilling at 0°C for up to 180 min. The effect on gene expression was also studied during a 180 min warming period after chilling for 30 or 60 min. There were significant decreases in sox2 (up to 4-fold) and sox3 (up to 3-fold) expressions following chilling. Significant increases in gene expressions of sox2 (up to 2-fold), sox3 (up to 33-fold) and sox19a (up to 25-fold) were observed during warming in the embryos that had been chilled for 30 min. Similarly, significant increases were observed in sox2 (up to 3-fold) and sox3 (up to 2-fold) during warming in embryos that had been chilled for 60 min. These increases may be explained by compensation for the suppression observed during chilling and/or to activate repair mechanisms or maintain homeostasis.Citation
Desai, K., Spikings, E., Zhang, T. (2011) 'Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos' Cryobiology 63 (2):96-103Publisher
ElsevierJournal
CryobiologyPubMed ID
21820425Additional Links
http://www.ncbi.nlm.nih.gov/pubmed/21820425http://www.sciencedirect.com/science/article/pii/S0011224011001167
Type
ArticleLanguage
enISSN
1090-2392ae974a485f413a2113503eed53cd6c53
10.1016/j.cryobiol.2011.07.001