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    Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants.

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    Authors
    Tsai, Sujune
    Spikings, Emma
    Huang, I-C.
    Lin, C.
    Affiliation
    Mingdao University
    Issue Date
    2011-01
    Subjects
    gorgonian coral
    oocyte
    mitochondrial membrane potential
    cryoprotectants
    
    Metadata
    Show full item record
    Abstract
    Coral reefs provide a valuable habitat for many economically valuable fish and invertebrates. However, they are in serious jeopardy, threatened by increasing over-exploitation, pollution, habitat destruction, disease and global climate change. Here, we examined the effect of cryoprotectant exposure on mitochondrial activity and membrane potential in coral oocytes in order to find suitable cryoprotectants towards their successful cryopreservation. According to the No Observed Effect Concentrations (NOECs), methanol was found to be the least toxic cryoprotectant whilst DMSO was the most toxic cryoprotectant. The results also demonstrated that there were no significant differences (p > 0.05) in ATP concentrations between Junceella juncea and Junceella fragilis after exposure to all concentrations of all cryoprotectants for 30 min. Using confocal microscopy, JC-1 (5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-imidacarbocyanine iodide) staining indicated that the mitochondrial membrane potential of Junceella fragilis oocytes reduced after 1 M and 2 M methanol treatment and a loss of the mitochondrial distribution pattern and poor green fluorescence after 3M methanol treatment. Therefore, even oocytes that show no adverse effect of cryoprotectants on survival might suffer some more subtle impacts. The results obtained from this study will provide a basis for development of protocols to cryopreserve the oocytes of gorgonian corals.
    Citation
    Tsai, S., Spikings, E., Huang, I-C., Lin, C. (2011) 'Study on the mitochondrial activity and membrane potential after exposing later stage oocytes of two gorgonian corals (Junceella juncea and Junceella fragilis) to cryoprotectants' Cryo Letters 32 (1):1-12
    Publisher
    Cryo Letters
    Journal
    Cryo letters
    URI
    http://hdl.handle.net/10547/228730
    PubMed ID
    21468448
    Additional Links
    http://www.ncbi.nlm.nih.gov/pubmed/21468448
    http://www.ingentaconnect.com/content/cryo/cryo/2011/00000032/00000001/art00001?token=00541bf705f864ec38341333c4a2f7a6c6a533847462148763b734f6d6222346b6268763050214d7767c
    Type
    Article
    Language
    en
    ISSN
    0143-2044
    Collections
    Cell and Cryobiology Research Group

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