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    Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres

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    Authors
    Lin, C.
    Spikings, Emma
    Zhang, Tiantian
    Rawson, David M.
    Affiliation
    University of Bedfordshire
    Issue Date
    2009-08
    Subjects
    zebrafish
    cryobiology
    embryos
    blastomeres
    gene expression
    pax
    chilling
    cryopreservation
    
    Metadata
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    Abstract
    Cryopreservation is now common practice in the fields of aquaculture, conservation and biomedicine. However, there is a lack of information on the effect of chilling and cryopreservation at the molecular level. In the present study, we used real-time RT-PCR analysis to determine the effect of chilling and cryopreservation on expression of Pax2a, Pax2b, Pax5 and Pax8 which constitute one subgroup of the Pax gene family. As intact embryos of zebrafish have not yet been successfully cryopreserved, we have used two alternatives: chilling of intact embryos and cryopreservation of isolated blastomeres. Cryopreservation was found to affect the normal pattern of gene expression in zebrafish embryonic blastomeres. The trends, profile changes, in expression of Pax2a and Pax5 occurred to a lesser extent in frozen-thawed blastomeres than in fresh blastomeres whilst the opposite was true for Pax8. The trends in expression of Pax2b were delayed in frozen-thawed blastomeres compared to fresh blastomeres. Cryopreservation can therefore disrupt normal gene expression patterns in zebrafish embryonic blastomeres which could have a detrimental effect on embryo development.
    Citation
    Lin, C., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres' Cryobiology 59 (1):42-47
    Publisher
    Elsevier
    Journal
    Cryobiology
    URI
    http://hdl.handle.net/10547/228724
    DOI
    10.1016/j.cryobiol.2009.04.007
    PubMed ID
    19397904
    Additional Links
    http://www.ncbi.nlm.nih.gov/pubmed/19397904
    http://www.sciencedirect.com/science/article/pii/S0011224009000376
    Type
    Article
    Language
    en
    ISSN
    1090-2392
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.cryobiol.2009.04.007
    Scopus Count
    Collections
    Cell and Cryobiology Research Group

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