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    Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres.

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    Authors
    Lin, C.
    Spikings, Emma
    Zhang, Tiantian
    Rawson, David M.
    Affiliation
    University of Bedfordshire
    Issue Date
    2009-04-15
    Subjects
    cryopreservation
    housekeeping gene
    embryos
    blastomeres
    zebrafish
    
    Metadata
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    Abstract
    Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.
    Citation
    Lin, C., Spikings, E., Zhang, T., Rawson, D.M. (2009) 'Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres' Theriogenology 71 (7):1147-1155
    Publisher
    Elsevier
    Journal
    Theriogenology
    URI
    http://hdl.handle.net/10547/228723
    DOI
    10.1016/j.theriogenology.2008.12.013
    PubMed ID
    19201018
    Additional Links
    http://www.ncbi.nlm.nih.gov/pubmed/19201018
    http://www.sciencedirect.com/science/article/pii/S0093691X08008054
    Type
    Article
    Language
    en
    ISSN
    0093-691X
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.theriogenology.2008.12.013
    Scopus Count
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    Cell and Cryobiology Research Group

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