Two closely related ABC transporters in streptococcus mutans are involved in disaccharide and/or oligosaccharide uptake.
AffiliationKing's College London
MetadataShow full item record
AbstractStreptococcus mutans has a large number of transporters apparently involved in the uptake of carbohydrates. At least two of these, the multiple sugar metabolism transporter, MsmEFGK, and the previously uncharacterized MalXFGK, are members of the ATP-binding cassette (ABC) superfamily. Mutation analysis revealed that the MsmEFGK and MalXFGK transporters are principally involved in the uptake of distinct disaccharides and/or oligosaccharides. Furthermore, the data also indicated an unusual protein interaction between the components of these two related transporters. Strains lacking msmE (which encodes a solute binding protein) can no longer utilize raffinose or stachyose but grow normally on maltodextrins in the absence of MalT, a previously characterized EII(mal) phosphotransferase system component. In contrast, a mutant of malX (which encodes a solute binding protein) cannot utilize maltodextrins but grows normally on raffinose or stachyose. Radioactive uptake assays confirmed that MalX, but not MsmE, is required for uptake of [U-14C]maltotriose and that MalXFGK is principally involved in the uptake of maltodextrins with as many as 7 glucose units. Surprisingly, inactivation of the corresponding ATPase components did not result in an equivalent abolition of growth: the malK mutant can grow on maltotetraose as a sole carbon source, and the msmK mutant can utilize raffinose. We propose that the ATPase domains of these ABC transporters can interact with either their own or the alternative transporter complex. Such unexpected interaction of ATPase subunits with distinct membrane components to form complete multiple ABC transporters may be widespread in bacteria.
CitationWebb, A.J., Homer, K.A., Hosie, A.H.F. (2008) 'Two closely related ABC transporters in streptococcus mutans are involved in disaccharide and/or oligosaccharide uptake', Journal of bacteriology 190 (1):168-78
PublisherAmerican Society for Microbiology
JournalJournal of bacteriology
PubMed Central IDPMC2223742
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/
- Global transcriptional analysis of Streptococcus mutans sugar transporters using microarrays.
- Authors: Ajdić D, Pham VT
- Issue date: 2007 Jul
- Enterococcus faecalis Uses a Phosphotransferase System Permease and a Host Colonization-Related ABC Transporter for Maltodextrin Uptake.
- Authors: Sauvageot N, Mokhtari A, Joyet P, Budin-Verneuil A, Blancato VS, Repizo GD, Henry C, Pikis A, Thompson J, Magni C, Hartke A, Deutscher J
- Issue date: 2017 May 1
- Identification of an ATPase, MsmK, which energizes multiple carbohydrate ABC transporters in Streptococcus pneumoniae.
- Authors: Marion C, Aten AE, Woodiga SA, King SJ
- Issue date: 2011 Oct
- A member of the second carbohydrate uptake subfamily of ATP-binding cassette transporters is responsible for ribonucleoside uptake in Streptococcus mutans.
- Authors: Webb AJ, Hosie AH
- Issue date: 2006 Dec
- Structural model of MalK, the ABC subunit of the maltose transporter of Escherichia coli: implications for mal gene regulation, inducer exclusion, and subunit assembly.
- Authors: Böhm A, Diez J, Diederichs K, Welte W, Boos W
- Issue date: 2002 Feb 1