The consequences of nuclear transfer for mammalian foetal development and offspring survival : a mitochondrial DNA perspective
Authors
St John, Justin C.Lloyd, Rhiannon E.I.
Bowles, Emma J.
Thomas, Emma C.
El Shourbagy, Shahinaz H.
Affiliation
University of BirminghamIssue Date
2004-06
Metadata
Show full item recordAbstract
The introduction of nuclear transfer (NT) and other technologies that involve embryo reconstruction require us to reinvestigate patterns of mitochondrial DNA (mtDNA) transmission, transcription and replication. MtDNA is a 16.6 kb genome located within each mitochondrion. The number of mitochondria and mtDNA copies per organelle is specific to each cell type. MtDNA is normally transmitted through the oocyte to the offspring. However, reconstructed oocytes often transmit both recipient oocyte mtDNA and mtDNA associated with the donor nucleus. We argue that the transmission of two populations of mtDNA may have implications for offspring survival as only one allele might be actively transcribed. This could result in the offspring phenotypically exhibiting mtDNA depletion-type syndromes. A similar occurrence could arise when nucleo-cytoplasmic interactions fail to regulate mtDNA transcription and replication, especially as the initiation of mtDNA replication post-implantation is a key developmental event. Furthermore, failure of the donor somatic nucleus to be reprogrammed could result in the early initiation of replication and the loss of cellular mtDNA specificity. We suggest investigations should be conducted to enhance our understanding of nucleo-cytoplasmic interactions in order to improve NT efficiency.Citation
St John, J.C., Lloyd, R.E.I., Bowles, E.J., Thomas, E.C., El Shourbagy, S.H. (2004) 'The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective', Reproduction 127 (6):631-41Publisher
Society for Reproduction and FertilityPubMed ID
15175500Additional Links
http://www.ncbi.nlm.nih.gov/pubmed/15175500Type
ArticleLanguage
enDescription
Review of the articleISSN
1470-1626ae974a485f413a2113503eed53cd6c53
10.1530/rep.1.00138
Scopus Count
Collections
The following license files are associated with this item:
Related articles
- Nuclear transfer: preservation of a nuclear genome at the expense of its associated mtDNA genome(s).
- Authors: Bowles EJ, Campbell KH, St John JC
- Issue date: 2007
- Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer.
- Authors: Spikings EC, Alderson J, St John JC
- Issue date: 2006 Jul-Aug
- Cytoplasmic inheritance and its effects on development and performance.
- Authors: Smith LC, Alcivar AA
- Issue date: 1993
- Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer.
- Authors: Burgstaller JP, Schinogl P, Dinnyes A, Müller M, Steinborn R
- Issue date: 2007 Dec 21
- Aberrant heteroplasmic transmission of mtDNA in cloned pigs arising from double nuclear transfer.
- Authors: St John JC, Moffatt O, D'Souza N
- Issue date: 2005 Dec