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    Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes

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    Authors
    Zampolla, Tiziana
    Spikings, Emma
    Rawson, David M.
    Zhang, Tiantian
    Affiliation
    University of Bedfordshire
    Issue Date
    2011-10-01
    Subjects
    cytoskeletonproteins
    zebrafish
    ovarian follicle
    mitochondria
    
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    Abstract
    The distributional arrangement of mitochondria in the granulosa cells surrounding stage III zebrafish oocyte has been reported as a contiguous aggregation of mitochondria at the margin of the each granulosa cell. The aim of the present study was to further investigate the mitochondrial distribution in the granulosa cell layer in stage III ovarian follicles and the interaction between mitochondria and cytoskeleton elements actin and tubulin. To determine mitochondrial distribution/transport, immunocytochemistry analysis of tubulin and mitochondrial COX-I was carried out along with phalloidin staining of polymerised F-actin. The follicles were also exposed to a range of conditions that are known to affect mitochondria and the cytoskeleton proteins actin and tubulin. The mitochondrial inhibitor FCCP, the anti-mitotic drug nocodazole, and actin polymerisation inhibitor cytochalasin B were used. Levels of ATP, mtDNA copy number, and viability assessed by Trypan blue were also studied after exposure to inhibitors in order to determine the relationship between mitochondrial distribution/activity and ATP production. F-actin showed a hexagonal-polygonal distribution surrounding the mitochondria in granulosa cells, with the F-actin network adjacent to the plasma membrane of each granulosa cell. Tubulin structure presented a less organised distribution than F-actin, it was sparse in the cytosol. Interaction between mitochondria and tubulin was found indicating that mitochondria and tubulin are colocalised in zebrafish ovarian follicles. The exposure of ovarian follicles to inhibitors induced the loss of mitochondrial structural integrity showing that mitochondria distribution in granulosa cells of stage III zebrafish ovarian follicles is determined by the microtubules network.
    Citation
    Zampolla, T., Spikings, E., Rawson, D.M., Zhang, T. (2011) 'Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes' Theriogenology 76 (6):1110-1119
    Publisher
    Elsevier
    Journal
    Theriogenology
    URI
    http://hdl.handle.net/10547/228293
    DOI
    10.1016/j.theriogenology.2011.05.018
    PubMed ID
    21752457
    Additional Links
    http://www.ncbi.nlm.nih.gov/pubmed/21752457
    http://www.sciencedirect.com/science/article/pii/S0093691X11002524
    Type
    Article
    Language
    en
    ISSN
    1879-3231
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.theriogenology.2011.05.018
    Scopus Count
    Collections
    Environmental Monitoring Research Group

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