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dc.contributor.authorLukman, Suryanien_GB
dc.contributor.authorGrant, Guy H.en_GB
dc.date.accessioned2012-06-01T13:46:29Z
dc.date.available2012-06-01T13:46:29Z
dc.date.issued2009-08-15
dc.identifier.citationLukman, S., Grant G.H. (2009) 'A network of dynamically conserved residues deciphers the motions of maltose transporter' Proteins 76 (3):588-97en_GB
dc.identifier.issn1097-0134
dc.identifier.pmid19274733
dc.identifier.doi10.1002/prot.22372
dc.identifier.urihttp://hdl.handle.net/10547/227130
dc.description.abstractThe maltose transporter of Escherichia coli is a member of the ATP-binding cassette (ABC) transporter superfamily. The crystal structures of maltose transporter MalK have been determined for distinct conformations in the presence and absence of the ligand ATP, and other interacting proteins. Using the distinct MalK structures, normal mode analysis was performed to understand the dynamics behavior of the system. A network of dynamically important residues was obtained from the normal mode analysis and the analysis of point mutation on the normal modes. Our results suggest that the intradomain rotation occurs earlier than the interdomain rotation during the maltose-binding protein (MBP)-driven conformational changes of MalK. We inquire if protein motion and functional-driven evolutionary conservation are related. The sequence conservation of MalK was analyzed to derive a network of evolutionarily important residues. There are highly significant correlations between protein sequence and protein dynamics in many regions on the maltose transporter MalK, suggesting a link between the protein evolution and dynamics. The significant overlaps between the network of dynamically important residues and the network of evolutionarily important residues form a network of dynamically conserved residues.
dc.language.isoenen
dc.publisherWiley-Blackwellen_GB
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/prot.22372/abstract;jsessionid=A6EBA2CF3DF51272983A9265E1620827.f01t04
dc.rightsArchived with thanks to Proteinsen_GB
dc.subject.meshATP-Binding Cassette Transporters
dc.subject.meshAmino Acid Sequence
dc.subject.meshEscherichia coli Proteins
dc.subject.meshEvolution, Molecular
dc.subject.meshMolecular Sequence Data
dc.subject.meshProtein Structure, Secondary
dc.subject.meshThermodynamics
dc.titleA network of dynamically conserved residues deciphers the motions of maltose transporteren
dc.typeArticleen
dc.contributor.departmentUniversity of Cambridgeen_GB
dc.identifier.journalProteinsen_GB
html.description.abstractThe maltose transporter of Escherichia coli is a member of the ATP-binding cassette (ABC) transporter superfamily. The crystal structures of maltose transporter MalK have been determined for distinct conformations in the presence and absence of the ligand ATP, and other interacting proteins. Using the distinct MalK structures, normal mode analysis was performed to understand the dynamics behavior of the system. A network of dynamically important residues was obtained from the normal mode analysis and the analysis of point mutation on the normal modes. Our results suggest that the intradomain rotation occurs earlier than the interdomain rotation during the maltose-binding protein (MBP)-driven conformational changes of MalK. We inquire if protein motion and functional-driven evolutionary conservation are related. The sequence conservation of MalK was analyzed to derive a network of evolutionarily important residues. There are highly significant correlations between protein sequence and protein dynamics in many regions on the maltose transporter MalK, suggesting a link between the protein evolution and dynamics. The significant overlaps between the network of dynamically important residues and the network of evolutionarily important residues form a network of dynamically conserved residues.


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