Environmental Science
http://hdl.handle.net/10547/556923
2024-03-29T06:01:11ZMolecular diversity of anthracnose pathogen populations associated with UK strawberry production suggests multiple introductions of three different Colletotrichum species.
http://hdl.handle.net/10547/576992
Molecular diversity of anthracnose pathogen populations associated with UK strawberry production suggests multiple introductions of three different Colletotrichum species.
Baroncelli, Riccardo; Zapparata, Antonio; Sarrocco, Sabrina; Sukno, Serenella A.; Lane, Charles R.; Thon, Michael R.; Vannacci, Giovanni; Holub, Eric; Sreenivasaprasad, Surapareddy
Fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging to Rosaceae family. Colletotrichum acutatum sensu lato (s.l.) is considered to be the second most economically important pathogen worldwide affecting strawberries. A collection of 148 Colletotrichum spp. isolates including 67 C. acutatum s.l. isolates associated with the phytosanitary history of UK strawberry production were used to characterize multi-locus genetic variation of this pathogen in the UK, relative to additional reference isolates that represent a worldwide sampling of the diversity of the fungus. The evidence indicates that three different species C. nymphaeae, C. godetiae and C. fioriniae are associated with strawberry production in the UK, which correspond to previously designated genetic groups A2, A4 and A3, respectively. Among these species, 12 distinct haplotypes were identified suggesting multiple introductions into the country. A subset of isolates was also used to compare aggressiveness in causing disease on strawberry plants and fruits. Isolates belonging to C. nymphaeae, C. godetiae and C. fioriniae representative of the UK anthracnose pathogen populations showed variation in their aggressiveness. Among the three species, C. nymphaeae and C. fioriniae appeared to be more aggressive compared to C. godetiae. This study highlights the genetic and pathogenic heterogeneity of the C. acutatum s.l. populations introduced into the UK linked to strawberry production.
2015-02-01T00:00:00ZVirulence diversity of anthracnose pathogens (Colletotrichum acutatum and C. gloeosporioides complexes) on eight olive cultivars commonly grown in Portugal.
http://hdl.handle.net/10547/576991
Virulence diversity of anthracnose pathogens (Colletotrichum acutatum and C. gloeosporioides complexes) on eight olive cultivars commonly grown in Portugal.
Talhinhas, Pedro; Gonçalves, Elsa; Sreenivasaprasad, Surapareddy; Oliveira, Helena
Olive anthracnose, caused by Colletotrichum acutatum and C. gloeosporioides species complexes, is a major disease affecting fruits at maturity, causing significant yield losses, and poor fruit and oil quality. Diverse genetic groups, particularly belonging to C. acutatum s.l. have been reported among the pathogens, with recent research proposing these genetic groups as distinct species. In this work, the virulence diversity of isolates representing different populations of C. acutatum s.l. and C. gloeosporioides s.s. was studied using a set of eight olive cultivars. Higher disease severity was produced by isolates belonging to groups A2 and A5 of C. acutatum s.l. (=C. nymphaeae and C. acutatum s.s., respectively) compared to C. gloeosporioides s.s. isolates as well as isolates of C. acutatum s.l. group A4 (=C. godetiae). Anthracnose severity was higher on the cultivars ‘Cobrançosa’, ‘Maçanilha de Tavira’ and ‘Galega Vulgar’ and lower in ‘Azeitoneira’, ‘Blanqueta’, ‘Negrinha de Freixo’ and ‘Picual’, but results indicate the occurrence of isolate × cultivar interactions. Differences in severity could be related to differences in conidia germination and appressoria formation, suggesting that early host-pathogen recognition events can in part explain disease severity under favourable environmental conditions. Overall results revealed the higher virulence and fitness levels of genotypes belonging to certain genetic groups within C. acutatum suggesting their ability to adapt to diverse agro-climatic conditions including specific hosts.
2015-05-01T00:00:00ZDraft genome sequence of Colletotrichum acutatum sensu lato (Colletotrichum fioriniae)
http://hdl.handle.net/10547/576990
Draft genome sequence of Colletotrichum acutatum sensu lato (Colletotrichum fioriniae)
Baroncelli, Riccardo; Sreenivasaprasad, Surapareddy; Sukno, Serenella A.; Thon, Michael R.; Holub, Eric
In addition to its economic impact, Colletotrichum acutatum sensu lato is an interesting model for molecular investigations due to the diversity of host-determined specialization and reproductive lifestyles within the species complex. The pathogen Colletotrichum fioriniae forms part of this species complex and causes anthracnose in a wide range of crops and wild plants worldwide. Some members of this species have also been reported to be entomopathogenic. Here, we report the draft genome sequence of a heterothallic reference isolate of C. fioriniae (strain PJ7). This sequence provides a range of new resources that serve as a useful platform for further research in the field.
2014-04-01T00:00:00ZFirst report of Colletotrichum acutatum sensu lato (Colletotrichum godetiae) causing anthracnose on grapevine (Vitis vinifera) in the United Kingdom
http://hdl.handle.net/10547/576987
First report of Colletotrichum acutatum sensu lato (Colletotrichum godetiae) causing anthracnose on grapevine (Vitis vinifera) in the United Kingdom
Baroncelli, Riccardo; Sreenivasaprasad, Surapareddy; Lane, Charles R.; Thon, Michael R.; Sukno, Serenella A.
In November 2009, twelve specimens of grapevine (Vitis vinifera) cv. Brant in the area of Penrith, Cumbria in the United Kingdom were showing necrotic black leaf spotting and abundant orange sporulation on the upper leaf surface. Infected plant material was submitted to the FERA (Food and Environment Research Agency) Plant Clinic for diagnosis. The tissue samples were surface sterilised for one minute in 20% sodium hypochlorite and cultured on potato dextrose agar. Monoconidial isolates grown at 25°C with a 12 hour light period had light grey cottony aerial mycelium with colour ranging from whitish to dark grey on the reverse side of the colony. The cultures had orange spores organised in small masses and dark melanised structures similar to acervuli. Disease symptoms and morphology suggested that a member of the genus Colletotrichum caused the disease. Given the fact that strains of Colletotrichum often belong to aggregates of species that can be difficult or impossible to distinguish morphologically, a representative isolate was sent to the University of Warwick for further analysis.
2014-06-01T00:00:00Z