Ferulic acid ameliorates pentylenetetrazol-induced seizures by reducing neuron cell death
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2019-08-05Subjects
ferulic acidepilepsy
spatial learning
memory capacity
apoptosis
oxidative stress
B120 Physiology
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Abstract: To investigate the neuroprotective effect of ferulic acid (FA) in a pentylenetetrazol (PTZ)-induced seizures rat model, the seizures behaviour, spatial learning ability and memory capability of the rats were assessed. Both the antioxidation and anti-apoptosis pathways were also investigated. In this study, male Wistar rats were randomly divided into 3 groups (n = 12 in each group). For 28 days, the rats were administered saline alone (i.p. normal saline, NS group), PTZ (40 mg/kg, i.p., PTZ group) once daily to induce seizures, or FA (i.p. 60 mg/kg) 20 min before being given PTZ (40 mg/kg, i.p., FA + PTZ group) to assess the neuroprotective effect of FA. The seizures behaviour of the rats was analysed with the Racine scale. The spatial learning and memory capacity of the rats were assessed by the Morris water maze test. The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured, and both in situ staining with the DNA-binding bisbenzimide Hoechst 33258 and TUNEL assays were used to assess apoptosis. Western blotting was used to further analyse the expression of Apaf-1, caspase-9, caspase-3, Bcl-2, Bid, Bax, cleaved caspase-3 and cytochrome c. The results showed that compared to the those of the PTZ group, FA pre-treatment significantly (p < 0.01) reduced the Racine scores starting at day 4, prolonged the latency of the onset of seizure at day 28, reduced the escape latency period starting at day 2, increased the frequency of crossing the platform location, increased the SOD activity, reduced the MDA content and apoptosis rate, and upregulated the Bcl-2 levels whilst downregulating the Bax, cytochrome c, Apaf-1, caspase-9, caspase-3, cleaved caspase-3 and Bid expression levels. This study demonstrated that pre-treatment with FA exerts strong neuroprotective effects by reducing seizures behaviour and by improving spatial learning ability and memory capacity. The neuroprotective effect may be a result of a reduction in neuron cell death that occurs via the antioxidative and anti-apoptotic pathways.Citation
Zhang SH, Liu DH, Hu QY, Zhu JL, Wang SQ, Zhou S (2019) 'Ferulic acid ameliorates pentylenetetrazol-induced seizures by reducing neuron cell death', Epilepsy Research, 156 (106183), pp.-.Publisher
ElsevierJournal
Epilepsy ResearchPubMed ID
31404716Additional Links
https://www.sciencedirect.com/science/article/pii/S0920121119302049Type
ArticleLanguage
enISSN
0920-1211ae974a485f413a2113503eed53cd6c53
10.1016/j.eplepsyres.2019.106183
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