Goat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cells

2.50
Hdl Handle:
http://hdl.handle.net/10547/623199
Title:
Goat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cells
Authors:
Ji, Xiaoxi; Xu, Weili ( 0000-0002-6786-4124 ) ; Cui, Jie; Ma, Ying ( 0000-0002-2641-7926 ) ; Zhou, Shaobo ( 0000-0001-5214-2973 )
Abstract:
Bovine milk fat globule membrane (MFGM) has shown many health benefits, however, there has not been much study on non-cattle MFGMs. The purpose of this study was to compare the anti-proliferation effects and investigate the mechanisms of MFGMs from bovine, goat, buffalo, yak and camel milk in HT-29 cells. Results showed that protein content in MFGM of yak milk is the highest among five MFGM. All MFGMs inhibited cellular proliferation which was in agreement with cell morphology and apoptosis. However, the number of cells in S-phase from 24 h to 72 h was increased significantly by treatment with goat, buffalo and bovine MFGMs (100 μg/mL), but not yak and camel. All MFGMs treatment significantly reduced the mitochondrial membrane potential (with an order of goat>buffalo>bovine>camel>yak) and Bcl-2 expression, but increased the expression of both Bax and Caspase-3. Taken together, the results indicate that all MFGMs, especially goat and buffalo MFGMs, showed better effects at inducing apoptosis and inhibition of the proliferation of HT-29 cells. The mechanism might be arresting the cell cycle at S phase, depolarization of mitochondrial membrane potential, down-regulation of Bcl-2 expression and increase of Bax and Caspase-3 expression.
Citation:
Ji XX, Xu WL, Cui J, Ma Y, Zhou S (2019) 'Goat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cells', Scientific Reports, 9 (), pp.2577-.
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
22-Feb-2019
URI:
http://hdl.handle.net/10547/623199
DOI:
10.1038/s41598-019-39546-y
Additional Links:
https://www.nature.com/articles/s41598-019-39546-y
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorJi, Xiaoxien
dc.contributor.authorXu, Weilien
dc.contributor.authorCui, Jieen
dc.contributor.authorMa, Yingen
dc.contributor.authorZhou, Shaoboen
dc.date.accessioned2019-03-01T10:44:02Z-
dc.date.available2019-03-01T10:44:02Z-
dc.date.issued2019-02-22-
dc.identifier.citationJi XX, Xu WL, Cui J, Ma Y, Zhou S (2019) 'Goat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cells', Scientific Reports, 9 (), pp.2577-.en
dc.identifier.issn2045-2322-
dc.identifier.doi10.1038/s41598-019-39546-y-
dc.identifier.urihttp://hdl.handle.net/10547/623199-
dc.description.abstractBovine milk fat globule membrane (MFGM) has shown many health benefits, however, there has not been much study on non-cattle MFGMs. The purpose of this study was to compare the anti-proliferation effects and investigate the mechanisms of MFGMs from bovine, goat, buffalo, yak and camel milk in HT-29 cells. Results showed that protein content in MFGM of yak milk is the highest among five MFGM. All MFGMs inhibited cellular proliferation which was in agreement with cell morphology and apoptosis. However, the number of cells in S-phase from 24 h to 72 h was increased significantly by treatment with goat, buffalo and bovine MFGMs (100 μg/mL), but not yak and camel. All MFGMs treatment significantly reduced the mitochondrial membrane potential (with an order of goat>buffalo>bovine>camel>yak) and Bcl-2 expression, but increased the expression of both Bax and Caspase-3. Taken together, the results indicate that all MFGMs, especially goat and buffalo MFGMs, showed better effects at inducing apoptosis and inhibition of the proliferation of HT-29 cells. The mechanism might be arresting the cell cycle at S phase, depolarization of mitochondrial membrane potential, down-regulation of Bcl-2 expression and increase of Bax and Caspase-3 expression.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttps://www.nature.com/articles/s41598-019-39546-yen
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectmilk fat globule membraneen
dc.subjectapoptosisen
dc.subjectC130 Cell Biologyen
dc.titleGoat and buffalo milk fat globule membranes exhibit better effects at inducing apoptosis and reduction the viability of HT-29 cellsen
dc.typeArticleen
dc.identifier.journalScientific Reportsen
dc.date.updated2019-03-01T10:41:38Z-
dc.description.noteopen access article-
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