The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis.

2.50
Hdl Handle:
http://hdl.handle.net/10547/622751
Title:
The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis.
Authors:
Chen, Daliang; Janganan, Thamarai K.; Chen, Gongyou; Marques, Everaldo R.; Kress, Marcia R.; Goldman, Gustavo H.; Walmsley, Adrian R. ( 0000-0002-5136-0511 ) ; Borges-Walmsley, Maria Ines
Abstract:
Paracoccidioides brasiliensis is a human pathogenic fungus that switches from a saprobic mycelium to a pathogenic yeast. Consistent with the morphological transition being regulated by the cAMP-signalling pathway, there is an increase in cellular cAMP levels both transiently at the onset (< 24 h) and progressively in the later stages (> 120 h) of the transition to the yeast form, and this transition can be modulated by exogenous cAMP. We have cloned the cyr1 gene encoding adenylate cyclase (AC) and established that its transcript levels correlate with cAMP levels. In addition, we have cloned the genes encoding three Galpha (Gpa1-3), Gbeta (Gpb1) and Ggamma (Gpg1) G proteins. Gpa1 and Gpb1 interact with one another and the N-terminus of AC, but neither Gpa2 nor Gpa3 interacted with Gpb1 or AC. The interaction of Gpa1 with Gpb1 was blocked by GTP, but its interaction with AC was independent of bound nucleotide. The transcript levels for gpa1, gpb1 and gpg1 were similar in mycelium, but there was a transient excess of gpb1 during the transition, and an excess of gpa1 in yeast. We have interpreted our findings in terms of a novel signalling mechanism in which the activity of AC is differentially modulated by Gpa1 and Gpb1 to maintain the signal over the 10 days needed for the morphological switch.
Affiliation:
University of Durham; Universidade de Sao Paulo
Citation:
Chen D, Janganan TK, Chen G, Marques ER, Kress, Goldman GH, Walmsley AR, Borges-Walmsley MI (2007) 'The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis.', Molecular Microbiology, 65 (3), pp.761-79.
Publisher:
Wiley
Journal:
Molecular Microbiology
Issue Date:
30-Aug-2007
URI:
http://hdl.handle.net/10547/622751
DOI:
10.1111/j.1365-2958.2007.05824.x
PubMed ID:
17635191
Additional Links:
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2958.2007.05824.x
Type:
Article
Language:
en
ISSN:
0950-382X
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Daliangen
dc.contributor.authorJanganan, Thamarai K.en
dc.contributor.authorChen, Gongyouen
dc.contributor.authorMarques, Everaldo R.en
dc.contributor.authorKress, Marcia R.en
dc.contributor.authorGoldman, Gustavo H.en
dc.contributor.authorWalmsley, Adrian R.en
dc.contributor.authorBorges-Walmsley, Maria Inesen
dc.date.accessioned2018-06-20T11:00:21Z-
dc.date.available2018-06-20T11:00:21Z-
dc.date.issued2007-08-30-
dc.identifier.citationChen D, Janganan TK, Chen G, Marques ER, Kress, Goldman GH, Walmsley AR, Borges-Walmsley MI (2007) 'The cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis.', Molecular Microbiology, 65 (3), pp.761-79.en
dc.identifier.issn0950-382X-
dc.identifier.pmid17635191-
dc.identifier.doi10.1111/j.1365-2958.2007.05824.x-
dc.identifier.urihttp://hdl.handle.net/10547/622751-
dc.description.abstractParacoccidioides brasiliensis is a human pathogenic fungus that switches from a saprobic mycelium to a pathogenic yeast. Consistent with the morphological transition being regulated by the cAMP-signalling pathway, there is an increase in cellular cAMP levels both transiently at the onset (< 24 h) and progressively in the later stages (> 120 h) of the transition to the yeast form, and this transition can be modulated by exogenous cAMP. We have cloned the cyr1 gene encoding adenylate cyclase (AC) and established that its transcript levels correlate with cAMP levels. In addition, we have cloned the genes encoding three Galpha (Gpa1-3), Gbeta (Gpb1) and Ggamma (Gpg1) G proteins. Gpa1 and Gpb1 interact with one another and the N-terminus of AC, but neither Gpa2 nor Gpa3 interacted with Gpb1 or AC. The interaction of Gpa1 with Gpb1 was blocked by GTP, but its interaction with AC was independent of bound nucleotide. The transcript levels for gpa1, gpb1 and gpg1 were similar in mycelium, but there was a transient excess of gpb1 during the transition, and an excess of gpa1 in yeast. We have interpreted our findings in terms of a novel signalling mechanism in which the activity of AC is differentially modulated by Gpa1 and Gpb1 to maintain the signal over the 10 days needed for the morphological switch.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2958.2007.05824.xen
dc.rightsYellow - can archive pre-print (ie pre-refereeing)-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectParacoccidioides brasiliensisen
dc.subjectcAMP pathwayen
dc.titleThe cAMP pathway is important for controlling the morphological switch to the pathogenic yeast form of Paracoccidioides brasiliensis.en
dc.typeArticleen
dc.contributor.departmentUniversity of Durhamen
dc.contributor.departmentUniversidade de Sao Pauloen
dc.identifier.journalMolecular Microbiologyen
dc.date.updated2018-06-20T09:02:45Z-
dc.description.noteOpen Access article-

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