Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.

2.50
Hdl Handle:
http://hdl.handle.net/10547/622746
Title:
Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.
Authors:
Janganan, Thamarai K.; Mullin, Nic; Tzokov, Svetomir B.; Stringer, Sandra; Fagan, Robert P.; Hobbs, Jamie K.; Moir, Anne; Bullough, Per A.
Abstract:
Clostridium sporogenes is a non-pathogenic close relative and surrogate for Group I (proteolytic) neurotoxin-producing Clostridium botulinum strains. The exosporium, the sac-like outermost layer of spores of these species, is likely to contribute to adhesion, dissemination, and virulence. A paracrystalline array, hairy nap, and several appendages were detected in the exosporium of C. sporogenes strain NCIMB 701792 by EM and AFM. The protein composition of purified exosporium was explored by LC-MS/MS of tryptic peptides from major individual SDS-PAGE-separated protein bands, and from bulk exosporium. Two high molecular weight protein bands both contained the same protein with a collagen-like repeat domain, the probable constituent of the hairy nap, as well as cysteine-rich proteins CsxA and CsxB. A third cysteine-rich protein (CsxC) was also identified. These three proteins are also encoded in C. botulinum Prevot 594, and homologues (75-100% amino acid identity) are encoded in many other Group I strains. This work provides the first insight into the likely composition and organization of the exosporium of Group I C. botulinum spores.
Affiliation:
Institute of Food Research; University of Sheffield
Citation:
Janganan TK, Mullin N, Tzokov SB, Stringer S, Fagan RP, Hobbs JK, Moir A, Bullough PA (2016) 'Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.', Food Microbiology, 59 (), pp.205-12.
Publisher:
Elsevier
Journal:
Food Microbiology
Issue Date:
6-Jun-2016
URI:
http://hdl.handle.net/10547/622746
DOI:
10.1016/j.fm.2016.06.003
PubMed ID:
27375261
Additional Links:
https://www.sciencedirect.com/science/article/pii/S0740002016302301
Type:
Article
Language:
en
ISSN:
0740-0020
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorJanganan, Thamarai K.en
dc.contributor.authorMullin, Nicen
dc.contributor.authorTzokov, Svetomir B.en
dc.contributor.authorStringer, Sandraen
dc.contributor.authorFagan, Robert P.en
dc.contributor.authorHobbs, Jamie K.en
dc.contributor.authorMoir, Anneen
dc.contributor.authorBullough, Per A.en
dc.date.accessioned2018-06-20T10:59:43Z-
dc.date.available2018-06-20T10:59:43Z-
dc.date.issued2016-06-06-
dc.identifier.citationJanganan TK, Mullin N, Tzokov SB, Stringer S, Fagan RP, Hobbs JK, Moir A, Bullough PA (2016) 'Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.', Food Microbiology, 59 (), pp.205-12.en
dc.identifier.issn0740-0020-
dc.identifier.pmid27375261-
dc.identifier.doi10.1016/j.fm.2016.06.003-
dc.identifier.urihttp://hdl.handle.net/10547/622746-
dc.description.abstractClostridium sporogenes is a non-pathogenic close relative and surrogate for Group I (proteolytic) neurotoxin-producing Clostridium botulinum strains. The exosporium, the sac-like outermost layer of spores of these species, is likely to contribute to adhesion, dissemination, and virulence. A paracrystalline array, hairy nap, and several appendages were detected in the exosporium of C. sporogenes strain NCIMB 701792 by EM and AFM. The protein composition of purified exosporium was explored by LC-MS/MS of tryptic peptides from major individual SDS-PAGE-separated protein bands, and from bulk exosporium. Two high molecular weight protein bands both contained the same protein with a collagen-like repeat domain, the probable constituent of the hairy nap, as well as cysteine-rich proteins CsxA and CsxB. A third cysteine-rich protein (CsxC) was also identified. These three proteins are also encoded in C. botulinum Prevot 594, and homologues (75-100% amino acid identity) are encoded in many other Group I strains. This work provides the first insight into the likely composition and organization of the exosporium of Group I C. botulinum spores.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0740002016302301en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectspore appendagesen
dc.subjecttwo dimensional crystalsen
dc.subjectcysteine rich proteinsen
dc.titleCharacterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.en
dc.typeArticleen
dc.contributor.departmentInstitute of Food Researchen
dc.contributor.departmentUniversity of Sheffielden
dc.identifier.journalFood Microbiologyen
dc.date.updated2018-06-20T00:00:20Z-

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