Milk fat globule membrane protein promotes C2C12 cell proliferation through the PI3K/Akt signaling pathway

2.50
Hdl Handle:
http://hdl.handle.net/10547/622670
Title:
Milk fat globule membrane protein promotes C2C12 cell proliferation through the PI3K/Akt signaling pathway
Authors:
Li, He; Xu, Weili ( 0000-0002-6786-4124 ) ; Ma, Ying ( 0000-0002-2641-7926 ) ; Zhou, Shaobo ( 0000-0001-5214-2973 ) ; Xiao, Ran
Abstract:
Milk fat globule membrane (MFGM) protein is known to have several health benefits, including an anti-sarcopenia effect; however, its mechanism is unclear. The aim of this study was to investigate the potential mechanism of action of the MFGM protein. The MFGM protein was extracted and separated into 4 fractions, and Fraction 2 (57 % of total MFGM) demonstrated the greatest effect on C2C12 cell proliferation. Milk fat globule-EGF factor 8 (MFG-E8) accounted for 82.35 % of the MFGM protein. The effects of whole Fraction 2 (100 μg/mL, 200 μg/mL and 300 μg/mL) on cell proliferation and morphology were measured. Using qRT-PCR or a Western blot assay, several regulatory factors, e.g., PI3K P85α, p-pI3K p85α (Tyr 508), Akt, p-Akt (Ser 473), mTOR and p-mTOR (Ser 2448), were measured in cells incubated with 200 μg/mL of Fraction 2 with or without wortmannin. The results demonstrated that Fraction 2 induced C2C12 cell proliferation in a dose-dependent manner, upregulated the mRNA expression of mTOR and p70S6K, and activated PI3K, Akt, mTOR and P70S6K phosphorylation; however, Fraction 2 inhibited FOXO3a and 4E-BP. The results demonstrate that the MFGM protein, predominantly MFG-E8, promotes cell proliferation through the PI3K/Akt/mTOR signaling pathway. This study elucidated the molecular mechanism of the MFGM protein, primarily MFG-E8, in promoting C2C12 cell proliferation via the PI3K/Akt/mTOR/P70S6K signal pathway.
Affiliation:
Harbin Institute of Technology; University of Bedfordshire
Citation:
Li H, Xu W, Ma Y, Zhou S, Xiao R. (2018) 'Milk fat globule membrane protein promotes C2C12 cell proliferation through the PI3K/Akt signaling pathway', International Journal of Biological Macromolecules, 114 (July), pp.1305-1314.
Publisher:
Elsevier
Journal:
International Journal of Biological Macromolecules
Issue Date:
7-Apr-2018
URI:
http://hdl.handle.net/10547/622670
DOI:
10.1016/j.ijbiomac.2018.04.026
Additional Links:
https://www.sciencedirect.com/science/article/pii/S0141813017352388?via=ihub
Type:
Article
Language:
en
ISSN:
0141-8130
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Heen
dc.contributor.authorXu, Weilien
dc.contributor.authorMa, Yingen
dc.contributor.authorZhou, Shaoboen
dc.contributor.authorXiao, Ranen
dc.date.accessioned2018-04-23T09:22:15Z-
dc.date.available2018-04-23T09:22:15Z-
dc.date.issued2018-04-07-
dc.identifier.citationLi H, Xu W, Ma Y, Zhou S, Xiao R. (2018) 'Milk fat globule membrane protein promotes C2C12 cell proliferation through the PI3K/Akt signaling pathway', International Journal of Biological Macromolecules, 114 (July), pp.1305-1314.en
dc.identifier.issn0141-8130-
dc.identifier.doi10.1016/j.ijbiomac.2018.04.026-
dc.identifier.urihttp://hdl.handle.net/10547/622670-
dc.description.abstractMilk fat globule membrane (MFGM) protein is known to have several health benefits, including an anti-sarcopenia effect; however, its mechanism is unclear. The aim of this study was to investigate the potential mechanism of action of the MFGM protein. The MFGM protein was extracted and separated into 4 fractions, and Fraction 2 (57 % of total MFGM) demonstrated the greatest effect on C2C12 cell proliferation. Milk fat globule-EGF factor 8 (MFG-E8) accounted for 82.35 % of the MFGM protein. The effects of whole Fraction 2 (100 μg/mL, 200 μg/mL and 300 μg/mL) on cell proliferation and morphology were measured. Using qRT-PCR or a Western blot assay, several regulatory factors, e.g., PI3K P85α, p-pI3K p85α (Tyr 508), Akt, p-Akt (Ser 473), mTOR and p-mTOR (Ser 2448), were measured in cells incubated with 200 μg/mL of Fraction 2 with or without wortmannin. The results demonstrated that Fraction 2 induced C2C12 cell proliferation in a dose-dependent manner, upregulated the mRNA expression of mTOR and p70S6K, and activated PI3K, Akt, mTOR and P70S6K phosphorylation; however, Fraction 2 inhibited FOXO3a and 4E-BP. The results demonstrate that the MFGM protein, predominantly MFG-E8, promotes cell proliferation through the PI3K/Akt/mTOR signaling pathway. This study elucidated the molecular mechanism of the MFGM protein, primarily MFG-E8, in promoting C2C12 cell proliferation via the PI3K/Akt/mTOR/P70S6K signal pathway.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0141813017352388?via=ihuben
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectsarcopeniaen
dc.subjectmilk fat globule membrane proteinen
dc.subjectC700 Molecular Biology, Biophysics and Biochemistryen
dc.titleMilk fat globule membrane protein promotes C2C12 cell proliferation through the PI3K/Akt signaling pathwayen
dc.typeArticleen
dc.contributor.departmentHarbin Institute of Technologyen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalInternational Journal of Biological Macromoleculesen
dc.date.updated2018-04-23T09:14:14Z-
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