Regulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsia

2.50
Hdl Handle:
http://hdl.handle.net/10547/622561
Title:
Regulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsia
Authors:
Jadoon, Ayesha; Cunningham, Phil; McDermott, Lindsay C.
Abstract:
Pre-eclampsia is characterized by placental hypoxia and dyslipidemia. Arachidonic and docosahexanoic acids are essential maternal nutrients for fetal development. They are transported via placental trophoblast cells by membrane and cytosolic fatty acid binding proteins. Others report the expressions of these proteins which are increased in hypoxic trophoblasts. Using bioinformatics, BeWo cells, reporter assays, quantitative real-time PCR and immunoblotting we tested the hypothesis that hypoxia inducible factors 1α (HIF-1α) and/ or 2α (HIF-2α) regulate the expressions of FABP1, FABP3, FABP4 and FATP2 proteins. Three hypoxia responsive elements (HRE) were identified in FABP1 which cumulatively responded strongly to HIF-1α and weakly to HIF-2α. FABP3 expression partially responded to HIF-1α. Two putative HRE were validated in FABP4 both of which responded weakly toHIF-1α and HIF-2α. FATP2 protein expression reacted positively to hypoxia. Thus, fetal essential fatty acid supply via the placenta is protected under hypoxia. It will be interesting to determine if our findings are replicated in human pre-eclamptic placenta.
Citation:
Jadoon A, Cunningham P, McDermott LC (2014) 'Regulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsia', Prostaglandins, Leukotrienes and Essential Fatty Acids, 93, pp.25-29.
Publisher:
Elsevier
Journal:
Prostaglandins, Leukotrienes and Essential Fatty Acids
Issue Date:
25-Sep-2014
URI:
http://hdl.handle.net/10547/622561
DOI:
10.1016/j.plefa.2014.09.004
Additional Links:
http://www.plefa.com/article/S0952-3278(14)00148-3/fulltext
Type:
Article
Language:
en
ISSN:
0952-3278
Sponsors:
EPSRC DTA award EP/P504961/1
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorJadoon, Ayeshaen
dc.contributor.authorCunningham, Philen
dc.contributor.authorMcDermott, Lindsay C.en
dc.date.accessioned2018-03-27T09:47:33Z-
dc.date.available2018-03-27T09:47:33Z-
dc.date.issued2014-09-25-
dc.identifier.citationJadoon A, Cunningham P, McDermott LC (2014) 'Regulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsia', Prostaglandins, Leukotrienes and Essential Fatty Acids, 93, pp.25-29.en
dc.identifier.issn0952-3278-
dc.identifier.doi10.1016/j.plefa.2014.09.004-
dc.identifier.urihttp://hdl.handle.net/10547/622561-
dc.description.abstractPre-eclampsia is characterized by placental hypoxia and dyslipidemia. Arachidonic and docosahexanoic acids are essential maternal nutrients for fetal development. They are transported via placental trophoblast cells by membrane and cytosolic fatty acid binding proteins. Others report the expressions of these proteins which are increased in hypoxic trophoblasts. Using bioinformatics, BeWo cells, reporter assays, quantitative real-time PCR and immunoblotting we tested the hypothesis that hypoxia inducible factors 1α (HIF-1α) and/ or 2α (HIF-2α) regulate the expressions of FABP1, FABP3, FABP4 and FATP2 proteins. Three hypoxia responsive elements (HRE) were identified in FABP1 which cumulatively responded strongly to HIF-1α and weakly to HIF-2α. FABP3 expression partially responded to HIF-1α. Two putative HRE were validated in FABP4 both of which responded weakly toHIF-1α and HIF-2α. FATP2 protein expression reacted positively to hypoxia. Thus, fetal essential fatty acid supply via the placenta is protected under hypoxia. It will be interesting to determine if our findings are replicated in human pre-eclamptic placenta.en
dc.description.sponsorshipEPSRC DTA award EP/P504961/1en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.plefa.com/article/S0952-3278(14)00148-3/fulltexten
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.subjectn-3 polyunsaturated fatty acidsen
dc.subjectpre-eclampsiaen
dc.subjectfatty acid binding proteinsen
dc.subjecthypoxiaen
dc.subjectlong chain polunsatured fatty acidsen
dc.subjecthypoxia-inducible factor-1 alphaen
dc.subjectplacentaen
dc.titleRegulation of fatty acid binding proteins by hypoxia inducible factors 1α and 2α in the placenta: Relevance to pre-eclampsiaen
dc.typeArticleen
dc.identifier.journalProstaglandins, Leukotrienes and Essential Fatty Acidsen
dc.date.updated2018-03-27T09:20:54Z-
dc.description.notebefore April 2016 so no file required-
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