Identification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sites

2.50
Hdl Handle:
http://hdl.handle.net/10547/622465
Title:
Identification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sites
Authors:
Wang, QingBiao; Xu, Yiqin; Gu, Zhuoya; Liu, Nian; Jin, Ke; Li, Yao; Crabbe, M. James C. ( 0000-0003-3609-1963 ) ; Zhong, Yang
Abstract:
Bacterial RNA polymerase (RNAP) is an effective target for antibacterial treatment. In order to search new potential targets in RNAP of Mycobacterium, we detected adaptive selections of RNAP related genes in 13 strains of Mycobacterium by phylogenetic analysis. We first collected sequences of 17 genes including rpoA, rpoB, rpoC, rpoZ, and sigma factor A-M. Then maximum likelihood trees were constructed, followed by positive selection detection. We found that sigG shows positive selection along the clade (M. tuberculosis, M. bovis), suggesting its important evolutionary role and its potential to be a new antibacterial target. Moreover, the regions near 933Cys and 935His on the rpoB subunit of M. tuberculosis showed significant positive selection, which could also be a new attractive target for anti-tuberculosis drugs.
Affiliation:
Fudan University; Oxford University; University of Bedfordshire; Tibet University
Citation:
Wang Q.B., Xu Y., Gu Z., Liu N., Jin K., Li Y., Crabbe,M.J.C., Zhong Y. (2018) 'Identification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sites', Computational Biology and Chemistry.
Publisher:
Elsevier
Journal:
Computational Biology and Chemistry
Issue Date:
21-Nov-2017
URI:
http://hdl.handle.net/10547/622465
DOI:
10.1016/j.compbiolchem.2017.11.002
Additional Links:
http://www.sciencedirect.com/science/article/pii/S1476927116303164
Type:
Article
Language:
en
ISSN:
1476-9271
EISSN:
1476-9271
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, QingBiaoen
dc.contributor.authorXu, Yiqinen
dc.contributor.authorGu, Zhuoyaen
dc.contributor.authorLiu, Nianen
dc.contributor.authorJin, Keen
dc.contributor.authorLi, Yaoen
dc.contributor.authorCrabbe, M. James C.en
dc.contributor.authorZhong, Yangen
dc.date.accessioned2018-01-19T14:26:44Z-
dc.date.available2018-01-19T14:26:44Z-
dc.date.issued2017-11-21-
dc.identifier.citationWang Q.B., Xu Y., Gu Z., Liu N., Jin K., Li Y., Crabbe,M.J.C., Zhong Y. (2018) 'Identification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sites', Computational Biology and Chemistry.en
dc.identifier.issn1476-9271-
dc.identifier.doi10.1016/j.compbiolchem.2017.11.002-
dc.identifier.urihttp://hdl.handle.net/10547/622465-
dc.description.abstractBacterial RNA polymerase (RNAP) is an effective target for antibacterial treatment. In order to search new potential targets in RNAP of Mycobacterium, we detected adaptive selections of RNAP related genes in 13 strains of Mycobacterium by phylogenetic analysis. We first collected sequences of 17 genes including rpoA, rpoB, rpoC, rpoZ, and sigma factor A-M. Then maximum likelihood trees were constructed, followed by positive selection detection. We found that sigG shows positive selection along the clade (M. tuberculosis, M. bovis), suggesting its important evolutionary role and its potential to be a new antibacterial target. Moreover, the regions near 933Cys and 935His on the rpoB subunit of M. tuberculosis showed significant positive selection, which could also be a new attractive target for anti-tuberculosis drugs.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1476927116303164en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectphylogenetic analysisen
dc.subjectselective pressureen
dc.subjectMycobacterium tuberculosisen
dc.titleIdentification of new antibacterial targets in RNA polymerase of Mycobacterium tuberculosis by detecting positive selection sitesen
dc.typeArticleen
dc.identifier.eissn1476-9271-
dc.contributor.departmentFudan Universityen
dc.contributor.departmentOxford Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.contributor.departmentTibet Universityen
dc.identifier.journalComputational Biology and Chemistryen
dc.date.updated2018-01-19T14:22:53Z-
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