Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum

2.50
Hdl Handle:
http://hdl.handle.net/10547/622283
Title:
Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum
Authors:
Chen, Haikui; Feng, Yang; Wang, Lina; Yonezawa, Takahiro; Crabbe, M. James C. ( 0000-0003-3609-1963 ) ; Zhang, Xiu; Zhong, Yang
Abstract:
Ultraviolet-B (UV-B) is a natural component of the solar radiation. Due to its high energy, low dosages of UV-B can bring huge potential damage effect to organisms. Despite much research that has analyzed the gene expression changes of plants that under UV-B radiation, the transcriptome response of Lycium ruthenicum under the UV-B induction is still unavailable. The aim of our study was to identify UV-B responsive genes and gain an insight into the underlying genetic basis of the pathobiology of UV-B related damage. We collected leaf samples from L. ruthenicum with and without UV-B exposure, and then performed a transcriptome profiling to comprehensively investigate their expression signatures. By employing the high throughput RNA-sequencing analysis of samples with and without UV-B exposure, we identified 1,913 up-regulated and 536 down-regulated genes at least by twofold changes. The activity of antioxidant enzyme related genes, including the superoxide dismutase, was decreased, genes related to the synthesis of secondary metabolites and defense responses, such as cinnamyl alcohol dehydrogenase, chalcone-flavanone isomerase and dihydroflavonol reductase were also downregulated. The expression patterns of 14 randomly selected genes resulted from quantitative real-time PCR were basically consistent with their transcript abundance changes identified by RNA-sequencing. We found that several biological pathways related to biotic and abiotic stresses, including cell defense, photosynthesis processes, energy metabolism, were involved in the process of UV-B stress response. A genome-wide screening of gene deregulation under UV-B induction would provide an insight into the understanding of the molecular bases and pathogenesis of UV-B responses.
Affiliation:
Beifang University of Nationalities, Yinchuan; Fudan University, Shanghai; University of Oxford; University of Bedfordshire; Tibet University
Citation:
Chen H., Feng Y., Wang L., Yonezawa T., Crabbe M.J.C., Zhang X., Zhong Y. (2015) 'Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum', Molecular Biology Reports, 42 (3), pp.639-649.
Publisher:
Springer
Journal:
Molecular Biology Reports
Issue Date:
15-Feb-2015
URI:
http://hdl.handle.net/10547/622283
DOI:
10.1007/s11033-014-3809-y
PubMed ID:
25366177
Additional Links:
https://link.springer.com/article/10.1007/s11033-014-3809-y
Type:
Article
Language:
en
ISSN:
0301-4851
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Haikuien
dc.contributor.authorFeng, Yangen
dc.contributor.authorWang, Linaen
dc.contributor.authorYonezawa, Takahiroen
dc.contributor.authorCrabbe, M. James C.en
dc.contributor.authorZhang, Xiuen
dc.contributor.authorZhong, Yangen
dc.date.accessioned2017-10-16T10:28:44Z-
dc.date.available2017-10-16T10:28:44Z-
dc.date.issued2015-02-15-
dc.identifier.citationChen H., Feng Y., Wang L., Yonezawa T., Crabbe M.J.C., Zhang X., Zhong Y. (2015) 'Transcriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicum', Molecular Biology Reports, 42 (3), pp.639-649.en
dc.identifier.issn0301-4851-
dc.identifier.pmid25366177-
dc.identifier.doi10.1007/s11033-014-3809-y-
dc.identifier.urihttp://hdl.handle.net/10547/622283-
dc.description.abstractUltraviolet-B (UV-B) is a natural component of the solar radiation. Due to its high energy, low dosages of UV-B can bring huge potential damage effect to organisms. Despite much research that has analyzed the gene expression changes of plants that under UV-B radiation, the transcriptome response of Lycium ruthenicum under the UV-B induction is still unavailable. The aim of our study was to identify UV-B responsive genes and gain an insight into the underlying genetic basis of the pathobiology of UV-B related damage. We collected leaf samples from L. ruthenicum with and without UV-B exposure, and then performed a transcriptome profiling to comprehensively investigate their expression signatures. By employing the high throughput RNA-sequencing analysis of samples with and without UV-B exposure, we identified 1,913 up-regulated and 536 down-regulated genes at least by twofold changes. The activity of antioxidant enzyme related genes, including the superoxide dismutase, was decreased, genes related to the synthesis of secondary metabolites and defense responses, such as cinnamyl alcohol dehydrogenase, chalcone-flavanone isomerase and dihydroflavonol reductase were also downregulated. The expression patterns of 14 randomly selected genes resulted from quantitative real-time PCR were basically consistent with their transcript abundance changes identified by RNA-sequencing. We found that several biological pathways related to biotic and abiotic stresses, including cell defense, photosynthesis processes, energy metabolism, were involved in the process of UV-B stress response. A genome-wide screening of gene deregulation under UV-B induction would provide an insight into the understanding of the molecular bases and pathogenesis of UV-B responses.en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urlhttps://link.springer.com/article/10.1007/s11033-014-3809-yen
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.subjectenvironmenten
dc.subjectLycium ruthenicumen
dc.subjectUV-Ben
dc.subjectD447 Environmental Conservationen
dc.titleTranscriptome profiling of the UV-B stress response in the desert shrub Lycium ruthenicumen
dc.typeArticleen
dc.contributor.departmentBeifang University of Nationalities, Yinchuanen
dc.contributor.departmentFudan University, Shanghaien
dc.contributor.departmentUniversity of Oxforden
dc.contributor.departmentUniversity of Bedfordshireen
dc.contributor.departmentTibet Universityen
dc.identifier.journalMolecular Biology Reportsen
dc.date.updated2017-10-16T09:59:13Z-
dc.description.noteCan't archive file as is publishers pdf, however, isn't needed as pre-April 2016, so passing metadata only. RVO 16/10/17-

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