Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons

2.50
Hdl Handle:
http://hdl.handle.net/10547/622234
Title:
Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons
Authors:
Wang, Shu-Qiu; Li, Xiao-Jie; Qiu, Hong-Bin; Jiang, Zhi-Mei; Simon, Maria; Ma, Xiao-Ru; Liu, Lei; Li, Jun-Xing; Wang, Fang-Fang; Liang, Yan-Feng; Wu, Jia-Mei; Di, Wei-Hua; Zhou, Shaobo ( 0000-0001-5214-2973 )
Abstract:
Purpose: To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II a expression in a model of epileptic neurons were investigated. Method: Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg2+ free medium for 3 hours; 3) Model group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg2+ free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II a protein expression was assessed by Western-blot. Ca2+ turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca2+ turnover was observed under a laser scanning confocal microscope. Results: The CaMK II a expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca2+ fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes. Conclusion: GLP may inhibit calcium overload and promote CaMK II a expression to protect epileptic neurons
Affiliation:
Jiamusi University; University of Bedfordshire
Citation:
Wang SQ, Li, XJ, Q HB, Jiang ZM, Simon M, Ma XR, Liu L, Li JX, Wang FF, LIang YF, Wu JM, Di WH, Zou S (2014) 'Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons', PLoS ONE, 9 (7).
Publisher:
PLOS
Journal:
PLoS ONE
Issue Date:
10-Jul-2014
URI:
http://hdl.handle.net/10547/622234
DOI:
10.1371/journal.pone.0102161
Additional Links:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102161
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Shu-Qiuen
dc.contributor.authorLi, Xiao-Jieen
dc.contributor.authorQiu, Hong-Binen
dc.contributor.authorJiang, Zhi-Meien
dc.contributor.authorSimon, Mariaen
dc.contributor.authorMa, Xiao-Ruen
dc.contributor.authorLiu, Leien
dc.contributor.authorLi, Jun-Xingen
dc.contributor.authorWang, Fang-Fangen
dc.contributor.authorLiang, Yan-Fengen
dc.contributor.authorWu, Jia-Meien
dc.contributor.authorDi, Wei-Huaen
dc.contributor.authorZhou, Shaoboen
dc.date.accessioned2017-09-25T11:39:07Z-
dc.date.available2017-09-25T11:39:07Z-
dc.date.issued2014-07-10-
dc.identifier.citationWang SQ, Li, XJ, Q HB, Jiang ZM, Simon M, Ma XR, Liu L, Li JX, Wang FF, LIang YF, Wu JM, Di WH, Zou S (2014) 'Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons', PLoS ONE, 9 (7).en
dc.identifier.issn1932-6203-
dc.identifier.doi10.1371/journal.pone.0102161-
dc.identifier.urihttp://hdl.handle.net/10547/622234-
dc.description.abstractPurpose: To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II a expression in a model of epileptic neurons were investigated. Method: Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg2+ free medium for 3 hours; 3) Model group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg2+ free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II a protein expression was assessed by Western-blot. Ca2+ turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca2+ turnover was observed under a laser scanning confocal microscope. Results: The CaMK II a expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca2+ fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes. Conclusion: GLP may inhibit calcium overload and promote CaMK II a expression to protect epileptic neuronsen
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102161en
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectepilepsyen
dc.subjectGanoderma lucidumen
dc.titleAnti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neuronsen
dc.typeArticleen
dc.contributor.departmentJiamusi Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalPLoS ONEen
dc.date.updated2017-09-25T11:16:36Z-
dc.description.noteopen access-
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