Antitumor effect of salidroside on mice bearing HepA hepatocellular carcinoma

2.50
Hdl Handle:
http://hdl.handle.net/10547/622232
Title:
Antitumor effect of salidroside on mice bearing HepA hepatocellular carcinoma
Authors:
Song, Hanjun; Wang, Jianjie; Wang, Molin; Dong, Hang; Li, Lijiang; Zhang, Tengyuan; Zhou, Shaobo ( 0000-0001-5214-2973 )
Abstract:
Salidroside, a phenylpropanoid glycoside extracted from Rhodiola rosea L., has antiproliferative effects on tumour cells in mice. However it’s antitumor mechanism remains largely unknown. In this study, 4 groups of mice bearing hepatocarcinoma cells were given treatment with vehicle alone, cyclophosphamide (25 mg/kg, i.p.) and salidroside, either 100 or 200 mg/kg (p.o.) for 14 days. The morphology of tumour specimens was analysed by transmission electron microscopy. Apoptotic cells in sections of mouse tumour tissue were analysed using an in situ apoptosis kit. The expression of Bcl-2, Bax and caspase 3 mRNA were examined with RT-PCR. The results showed that the tumour weights in groups 100 or 200 mg/kg/day of salidroside were reduced significantly (45.34 and 52.48% respectively), compared to vehicle groups. Salidroside increased apoptotic cells index, e.g. in 200 mg/kg group, it was four times higher compared to the control group. Even more, treatment with salidroside decreased Bcl-2 mRNA expression and increased Bax and caspase 3 mRNA expressions. These indicated that the antitumor mechanism of salidroside may induce tumour cell apoptosis in mice by triggering the mitochondrial-dependent pathway and activation of caspase 3.
Affiliation:
Jiamusi University; University of Bedfordshire
Citation:
Song H, Wand J, Wang M , Dong H, Li L, Zhang T, Zhoud S (2015) 'Antitumor effect of salidroside on mice bearing HepA hepatocellular carcinoma', Acta Farmaceutica Bonaerense, 34 (10).
Journal:
Acta Farmaceutica Bonaerense
Issue Date:
5-Aug-2015
URI:
http://hdl.handle.net/10547/622232
Additional Links:
http://www.latamjpharm.org/resumenes/34/10/LAJOP_34_10_1_8.pdf
Type:
Article
Language:
en
ISSN:
0326-2383
Appears in Collections:
Biomedical and biological science

Full metadata record

DC FieldValue Language
dc.contributor.authorSong, Hanjunen
dc.contributor.authorWang, Jianjieen
dc.contributor.authorWang, Molinen
dc.contributor.authorDong, Hangen
dc.contributor.authorLi, Lijiangen
dc.contributor.authorZhang, Tengyuanen
dc.contributor.authorZhou, Shaoboen
dc.date.accessioned2017-09-25T11:38:57Z-
dc.date.available2017-09-25T11:38:57Z-
dc.date.issued2015-08-05-
dc.identifier.citationSong H, Wand J, Wang M , Dong H, Li L, Zhang T, Zhoud S (2015) 'Antitumor effect of salidroside on mice bearing HepA hepatocellular carcinoma', Acta Farmaceutica Bonaerense, 34 (10).en
dc.identifier.issn0326-2383-
dc.identifier.urihttp://hdl.handle.net/10547/622232-
dc.description.abstractSalidroside, a phenylpropanoid glycoside extracted from Rhodiola rosea L., has antiproliferative effects on tumour cells in mice. However it’s antitumor mechanism remains largely unknown. In this study, 4 groups of mice bearing hepatocarcinoma cells were given treatment with vehicle alone, cyclophosphamide (25 mg/kg, i.p.) and salidroside, either 100 or 200 mg/kg (p.o.) for 14 days. The morphology of tumour specimens was analysed by transmission electron microscopy. Apoptotic cells in sections of mouse tumour tissue were analysed using an in situ apoptosis kit. The expression of Bcl-2, Bax and caspase 3 mRNA were examined with RT-PCR. The results showed that the tumour weights in groups 100 or 200 mg/kg/day of salidroside were reduced significantly (45.34 and 52.48% respectively), compared to vehicle groups. Salidroside increased apoptotic cells index, e.g. in 200 mg/kg group, it was four times higher compared to the control group. Even more, treatment with salidroside decreased Bcl-2 mRNA expression and increased Bax and caspase 3 mRNA expressions. These indicated that the antitumor mechanism of salidroside may induce tumour cell apoptosis in mice by triggering the mitochondrial-dependent pathway and activation of caspase 3.en
dc.language.isoenen
dc.relation.urlhttp://www.latamjpharm.org/resumenes/34/10/LAJOP_34_10_1_8.pdfen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectantitumoren
dc.subjectsalidrosideen
dc.titleAntitumor effect of salidroside on mice bearing HepA hepatocellular carcinomaen
dc.typeArticleen
dc.contributor.departmentJiamusi Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalActa Farmaceutica Bonaerenseen
dc.date.updated2017-09-25T11:16:39Z-
dc.description.noteno information in Sherpa and unable to find contact details for publisher. As available free online will assume full text OK unless told otherwise by publisher. RVO 25-9-17-
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