Determining the effect of temperature on species interactions in microcosms: a QPCR approach

2.50
Hdl Handle:
http://hdl.handle.net/10547/622104
Title:
Determining the effect of temperature on species interactions in microcosms: a QPCR approach
Authors:
Dowdeswell, Emily
Abstract:
Predicting the impact of environmental change is a major goal and challenge in ecology. With climate change threatening the biodiversity and ecosystem functioning of our natural ecosystems, understanding the effects of such change and how these are mediated through a community is of critical importance. Community stability could be severely affected by temperature through extinctions, alterations in species dominance and species-specific responses. One approach to testing the consequences of climate change on a community is to manipulate experimental aquatic microcosms. However, investigating community-level responses to change in experimental microcosms has been limited by the ability to accurately monitor the basal trophic level of bacteria. Here I develop a molecular approach to monitoring bacteria by using qPCR. The qPCR approach was successful for three bacterial species and produced sensitivity to the single cell. The qPCR approach was implemented in an experimental setting to aid the investigation of the relationships between temperature, species interactions and community properties. Direct temperature response was species-specific, but indirect interactions strongly mediated temperature through the community, altering competitor and predator response. Therefore, predicting species and community response to environmental change is dependent on knowledge of specific-species response, indirect pathways of interaction and the effects of community composition.
Citation:
Dowdeswell, E. (2016) 'Determining the effect of temperature on species interactions in microcosms: a QPCR approach'. MRes thesis. University of Bedfordshire.
Publisher:
University of Bedfordshire
Issue Date:
Jun-2016
URI:
http://hdl.handle.net/10547/622104
Type:
Thesis or dissertation
Language:
en
Description:
A thesis submitted to the Institute of Biomedical, Environmental Science and Technology, University of Bedfordshire, in fulfilment of the requirements for the degree of Masters by Research.
Appears in Collections:
Masters e-theses

Full metadata record

DC FieldValue Language
dc.contributor.authorDowdeswell, Emilyen
dc.date.accessioned2017-05-22T10:39:40Z-
dc.date.available2017-05-22T10:39:40Z-
dc.date.issued2016-06-
dc.identifier.citationDowdeswell, E. (2016) 'Determining the effect of temperature on species interactions in microcosms: a QPCR approach'. MRes thesis. University of Bedfordshire.en
dc.identifier.urihttp://hdl.handle.net/10547/622104-
dc.descriptionA thesis submitted to the Institute of Biomedical, Environmental Science and Technology, University of Bedfordshire, in fulfilment of the requirements for the degree of Masters by Research.en
dc.description.abstractPredicting the impact of environmental change is a major goal and challenge in ecology. With climate change threatening the biodiversity and ecosystem functioning of our natural ecosystems, understanding the effects of such change and how these are mediated through a community is of critical importance. Community stability could be severely affected by temperature through extinctions, alterations in species dominance and species-specific responses. One approach to testing the consequences of climate change on a community is to manipulate experimental aquatic microcosms. However, investigating community-level responses to change in experimental microcosms has been limited by the ability to accurately monitor the basal trophic level of bacteria. Here I develop a molecular approach to monitoring bacteria by using qPCR. The qPCR approach was successful for three bacterial species and produced sensitivity to the single cell. The qPCR approach was implemented in an experimental setting to aid the investigation of the relationships between temperature, species interactions and community properties. Direct temperature response was species-specific, but indirect interactions strongly mediated temperature through the community, altering competitor and predator response. Therefore, predicting species and community response to environmental change is dependent on knowledge of specific-species response, indirect pathways of interaction and the effects of community composition.en
dc.language.isoenen
dc.publisherUniversity of Bedfordshireen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectqPCRen
dc.subjectclimate changeen
dc.subjectenvironmental changeen
dc.subjecttemperature, species interactions and community propertiesen
dc.subjectspecies and community responseen
dc.subjectspecific-species responseen
dc.subjectcommunity compositionen
dc.titleDetermining the effect of temperature on species interactions in microcosms: a QPCR approachen
dc.typeThesis or dissertationen
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