The development of a novel on-line system for the monitoring and control of fermentation processes

2.50
Hdl Handle:
http://hdl.handle.net/10547/610796
Title:
The development of a novel on-line system for the monitoring and control of fermentation processes
Authors:
Wang, Yu
Abstract:
This thesis describes the development of a computer controlled on-line system for fermentation monitoring and control. The entire system consists of a laboratory fermenter, flow injection system (four channels), a newly designed on-line filter, biomass analysis channel, pH and oxygen controllers as well as a spectrophotometer. A new design of gas driven flow injection analysis (FIA) allows a large number of reagents to be handled. The computer-controlled four channel PIA system is well suited for sequential analysis, which is important for fermentation on-line mOnitoring. The system can change the wavelength of the spectrophotometer automatically for each PIA channel, which makes the system powerful and flexible. A high frequency, low energy ultrasonic filter was modified and applied to the system for on-line mammalian cell culture sampling without breaking the sterile barrier. The results show that this novel application of ultrasonic filter technology results in higher efficiency and reliability and a longer life cycle than other types of filter. All the operations of the analytical system are controlled by a Macintosh computer (Quadra 950). The control program was written in LabVIEW which is a graphical programming language and well applicable to fermentation control. The software communicates with detectors, data acquisition, data analysis and presentation. The system can programmatically control up to 50 devices. Mammalian cell batch culture was used as an example of the application of the system. The system consists of a laboratory fermenter with a continuous sample withdrawal filter and an analysis system where glucose, lactate and ammonia, lactate dehydrogenase and biomass were measured. Cell viability was estimated by microscopic assay with trypan blue. pH and Oxygen were also measured. The system response was fast and yields a large number of reliable and precise analytical results which can be of great importance in the monitoring and control of mammalian cell culture conditions.
Citation:
Wang, Y. (1995) 'The development of a novel on-line system for the monitoring and control of fermentation processes'. PhD Thesis. University of Luton.
Publisher:
University of Bedfordshire
Issue Date:
Jul-1995
URI:
http://hdl.handle.net/10547/610796
Type:
Thesis or dissertation
Language:
en
Description:
A thesis submitted to the University of Luton for the degree of Doctor of Philosophy
Appears in Collections:
PhD e-theses

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Yuen
dc.date.accessioned2016-05-26T12:18:50Zen
dc.date.available2016-05-26T12:18:50Zen
dc.date.issued1995-07en
dc.identifier.citationWang, Y. (1995) 'The development of a novel on-line system for the monitoring and control of fermentation processes'. PhD Thesis. University of Luton.en
dc.identifier.urihttp://hdl.handle.net/10547/610796en
dc.descriptionA thesis submitted to the University of Luton for the degree of Doctor of Philosophyen
dc.description.abstractThis thesis describes the development of a computer controlled on-line system for fermentation monitoring and control. The entire system consists of a laboratory fermenter, flow injection system (four channels), a newly designed on-line filter, biomass analysis channel, pH and oxygen controllers as well as a spectrophotometer. A new design of gas driven flow injection analysis (FIA) allows a large number of reagents to be handled. The computer-controlled four channel PIA system is well suited for sequential analysis, which is important for fermentation on-line mOnitoring. The system can change the wavelength of the spectrophotometer automatically for each PIA channel, which makes the system powerful and flexible. A high frequency, low energy ultrasonic filter was modified and applied to the system for on-line mammalian cell culture sampling without breaking the sterile barrier. The results show that this novel application of ultrasonic filter technology results in higher efficiency and reliability and a longer life cycle than other types of filter. All the operations of the analytical system are controlled by a Macintosh computer (Quadra 950). The control program was written in LabVIEW which is a graphical programming language and well applicable to fermentation control. The software communicates with detectors, data acquisition, data analysis and presentation. The system can programmatically control up to 50 devices. Mammalian cell batch culture was used as an example of the application of the system. The system consists of a laboratory fermenter with a continuous sample withdrawal filter and an analysis system where glucose, lactate and ammonia, lactate dehydrogenase and biomass were measured. Cell viability was estimated by microscopic assay with trypan blue. pH and Oxygen were also measured. The system response was fast and yields a large number of reliable and precise analytical results which can be of great importance in the monitoring and control of mammalian cell culture conditions.en
dc.language.isoenen
dc.publisherUniversity of Bedfordshireen
dc.subjectC790 Molecular Biology, Biophysics and Biochemistry not elsewhere classifieden
dc.subjectfermentationen
dc.subjectmonitoringen
dc.subjectflow injection analysisen
dc.subjectcell cultureen
dc.titleThe development of a novel on-line system for the monitoring and control of fermentation processesen
dc.typeThesis or dissertationen
dc.type.qualificationnamePhDen_GB
dc.type.qualificationlevelPhDen
dc.publisher.institutionUniversity of Bedfordshireen
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