2.50
Hdl Handle:
http://hdl.handle.net/10547/593507
Title:
Transferrin receptor 2 is crucial for iron sensing in human hepatocytes
Authors:
Rapisarda, Chiara; Puppi, Juliana; Hughes, Robin D.; Dhawan, Anil; Farnaud, Sébastien; Evans, Robert W.; Sharp, Paul A.
Abstract:
Hepcidin expression in vivo is regulated in proportion to iron status (i.e., increased by iron loading and decreased in iron deficiency). However, in vitro studies with hepatoma cell lines often show an inverse relationship between iron status and hepcidin expression. Here, we investigated possible molecular mechanisms responsible for the differences in iron sensing between hepatoma cell lines and human primary hepatocytes. RNA was collected from primary human hepatocytes, and HepG2 and HuH7 hepatoma cells were treated with either transferrin-bound and non-transferrin-bound iron. Expression of hepcidin, transferrin receptor 2, HFE, and hemojuvelin were quantified by real-time PCR. Hepcidin expression was increased in primary human hepatocytes following 24-h exposure to holoferric transferrin. In contrast, hepcidin mRNA levels in hepatoma cells were decreased by transferrin. Hepcidin expression was positively correlated with transferrin receptor 2 mRNA levels in primary human hepatocytes. Compared with primary hepatocytes, transferrin receptor 2 expression was significantly lower in hepatoma cell lines; furthermore, there was no correlation between transferrin receptor 2 and hepcidin mRNA levels in either HepG2 or HuH7 cells. Taken together our data suggest that transferrin receptor 2 is a likely candidate to explain the differences in iron sensing between hepatoma cell lines and primary human hepatocytes.
Citation:
Rapisarda, C. et al (2010) 'Transferrin receptor 2 is crucial for iron sensing in human hepatocytes' AJP: Gastrointestinal and Liver Physiology 299 (3):G778
Publisher:
American Physiological Society
Journal:
AJP: Gastrointestinal and Liver Physiology
Issue Date:
24-Jun-2010
URI:
http://hdl.handle.net/10547/593507
DOI:
10.1152/ajpgi.00157.2010
PubMed ID:
20576915
PubMed Central ID:
PMC2950680
Additional Links:
http://ajpgi.physiology.org/cgi/doi/10.1152/ajpgi.00157.2010; http://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20576915/
Type:
Article
Language:
en
ISSN:
0193-1857; 1522-1547
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorRapisarda, Chiaraen
dc.contributor.authorPuppi, Julianaen
dc.contributor.authorHughes, Robin D.en
dc.contributor.authorDhawan, Anilen
dc.contributor.authorFarnaud, Sébastienen
dc.contributor.authorEvans, Robert W.en
dc.contributor.authorSharp, Paul A.en
dc.date.accessioned2016-01-15T11:00:56Zen
dc.date.available2016-01-15T11:00:56Zen
dc.date.issued2010-06-24en
dc.identifier.citationRapisarda, C. et al (2010) 'Transferrin receptor 2 is crucial for iron sensing in human hepatocytes' AJP: Gastrointestinal and Liver Physiology 299 (3):G778en
dc.identifier.issn0193-1857en
dc.identifier.issn1522-1547en
dc.identifier.pmid20576915en
dc.identifier.doi10.1152/ajpgi.00157.2010en
dc.identifier.urihttp://hdl.handle.net/10547/593507en
dc.description.abstractHepcidin expression in vivo is regulated in proportion to iron status (i.e., increased by iron loading and decreased in iron deficiency). However, in vitro studies with hepatoma cell lines often show an inverse relationship between iron status and hepcidin expression. Here, we investigated possible molecular mechanisms responsible for the differences in iron sensing between hepatoma cell lines and human primary hepatocytes. RNA was collected from primary human hepatocytes, and HepG2 and HuH7 hepatoma cells were treated with either transferrin-bound and non-transferrin-bound iron. Expression of hepcidin, transferrin receptor 2, HFE, and hemojuvelin were quantified by real-time PCR. Hepcidin expression was increased in primary human hepatocytes following 24-h exposure to holoferric transferrin. In contrast, hepcidin mRNA levels in hepatoma cells were decreased by transferrin. Hepcidin expression was positively correlated with transferrin receptor 2 mRNA levels in primary human hepatocytes. Compared with primary hepatocytes, transferrin receptor 2 expression was significantly lower in hepatoma cell lines; furthermore, there was no correlation between transferrin receptor 2 and hepcidin mRNA levels in either HepG2 or HuH7 cells. Taken together our data suggest that transferrin receptor 2 is a likely candidate to explain the differences in iron sensing between hepatoma cell lines and primary human hepatocytes.en
dc.language.isoenen
dc.publisherAmerican Physiological Societyen
dc.relation.urlhttp://ajpgi.physiology.org/cgi/doi/10.1152/ajpgi.00157.2010en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20576915/en
dc.rightsArchived with thanks to AJP: Gastrointestinal and Liver Physiologyen
dc.subjecttransferrinen
dc.subjecthepcidinsen
dc.subjectiron deficiencyen
dc.subjecthepatocytesen
dc.titleTransferrin receptor 2 is crucial for iron sensing in human hepatocytesen
dc.typeArticleen
dc.identifier.journalAJP: Gastrointestinal and Liver Physiologyen
dc.identifier.pmcidPMC2950680en

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