Intervention effects of Ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of Neurotrophin-4 and N-Cadherin

2.50
Hdl Handle:
http://hdl.handle.net/10547/593453
Title:
Intervention effects of Ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of Neurotrophin-4 and N-Cadherin
Authors:
Wang, Shu-Qiu; Li, Xiao-Jie; Zhou, Shaobo ( 0000-0001-5214-2973 ) ; Sun, Di-Xiang; Wang, Hui; Cheng, Peng-Fei; Ma, Xiao-Ru; Liu, Lei; Liu, Jun-Xing; Wang, Fang-Fang; Liang, Yan-Feng; Wu, Jia-Mei
Abstract:
Epilepsy can cause cerebral transient dysfunctions. Ganoderma lucidum spores (GLS), a traditional Chinese medicinal herb, has shown some antiepileptic effects in our previous studies. This was the first study of the effects of GLS on cultured primary hippocampal neurons, treated with Mg2+ free medium. This in vitro model of epileptiform discharge hippocampal neurons allowed us to investigate the anti-epileptic effects and mechanism of GLS activity. Primary hippocampal neurons from <1 day old rats were cultured and their morphologies observed under fluorescence microscope. Neurons were confirmed by immunofluorescent staining of neuron specific enolase (NSE). Sterile method for GLS generation was investigated and serial dilutions of GLS were used to test the maximum non-toxic concentration of GLS on hippocampal neurons. The optimized concentration of GLS of 0.122 mg/ml was identified and used for subsequent analysis. Using the in vitro model, hippocampal neurons were divided into 4 groups for subsequent treatment i) control, ii) model (incubated with Mg2+ free medium for 3 hours), iii) GLS group I (incubated with Mg2+ free medium containing GLS for 3 hours and replaced with normal medium and incubated for 6 hours) and iv) GLS group II (neurons incubated with Mg2+ free medium for 3 hours then replaced with a normal medium containing GLS for 6 hours). Neurotrophin-4 and N-Cadherin protein expression were detected using Western blot. The results showed that the number of normal hippocampal neurons increased and the morphologies of hippocampal neurons were well preserved after GLS treatment. Furthermore, the expression of neurotrophin-4 was significantly increased while the expression of N-Cadherin was decreased in the GLS treated group compared with the model group. This data indicates that GLS may protect hippocampal neurons by promoting neurotrophin-4 expression and inhibiting N-Cadherin expression.
Affiliation:
Jiamusi University; University of Bedfordshire
Citation:
Wang, S-Q. et al (2013) 'Intervention Effects of Ganoderma Lucidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin-4 and N-Cadherin' PLoS ONE 8 (4):e61687
Publisher:
PLoS
Journal:
PLoS ONE
Issue Date:
24-Apr-2013
URI:
http://hdl.handle.net/10547/593453
DOI:
10.1371/journal.pone.0061687
PubMed ID:
23637882
PubMed Central ID:
PMC3634853
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0061687
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Shu-Qiuen
dc.contributor.authorLi, Xiao-Jieen
dc.contributor.authorZhou, Shaoboen
dc.contributor.authorSun, Di-Xiangen
dc.contributor.authorWang, Huien
dc.contributor.authorCheng, Peng-Feien
dc.contributor.authorMa, Xiao-Ruen
dc.contributor.authorLiu, Leien
dc.contributor.authorLiu, Jun-Xingen
dc.contributor.authorWang, Fang-Fangen
dc.contributor.authorLiang, Yan-Fengen
dc.contributor.authorWu, Jia-Meien
dc.date.accessioned2016-01-14T13:06:25Zen
dc.date.available2016-01-14T13:06:25Zen
dc.date.issued2013-04-24en
dc.identifier.citationWang, S-Q. et al (2013) 'Intervention Effects of Ganoderma Lucidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin-4 and N-Cadherin' PLoS ONE 8 (4):e61687en
dc.identifier.issn1932-6203en
dc.identifier.pmid23637882en
dc.identifier.doi10.1371/journal.pone.0061687en
dc.identifier.urihttp://hdl.handle.net/10547/593453en
dc.description.abstractEpilepsy can cause cerebral transient dysfunctions. Ganoderma lucidum spores (GLS), a traditional Chinese medicinal herb, has shown some antiepileptic effects in our previous studies. This was the first study of the effects of GLS on cultured primary hippocampal neurons, treated with Mg2+ free medium. This in vitro model of epileptiform discharge hippocampal neurons allowed us to investigate the anti-epileptic effects and mechanism of GLS activity. Primary hippocampal neurons from <1 day old rats were cultured and their morphologies observed under fluorescence microscope. Neurons were confirmed by immunofluorescent staining of neuron specific enolase (NSE). Sterile method for GLS generation was investigated and serial dilutions of GLS were used to test the maximum non-toxic concentration of GLS on hippocampal neurons. The optimized concentration of GLS of 0.122 mg/ml was identified and used for subsequent analysis. Using the in vitro model, hippocampal neurons were divided into 4 groups for subsequent treatment i) control, ii) model (incubated with Mg2+ free medium for 3 hours), iii) GLS group I (incubated with Mg2+ free medium containing GLS for 3 hours and replaced with normal medium and incubated for 6 hours) and iv) GLS group II (neurons incubated with Mg2+ free medium for 3 hours then replaced with a normal medium containing GLS for 6 hours). Neurotrophin-4 and N-Cadherin protein expression were detected using Western blot. The results showed that the number of normal hippocampal neurons increased and the morphologies of hippocampal neurons were well preserved after GLS treatment. Furthermore, the expression of neurotrophin-4 was significantly increased while the expression of N-Cadherin was decreased in the GLS treated group compared with the model group. This data indicates that GLS may protect hippocampal neurons by promoting neurotrophin-4 expression and inhibiting N-Cadherin expression.en
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0061687en
dc.rightsArchived with thanks to PLoS ONEen
dc.subjectepilepsyen
dc.subjectGanoderma lucidum sporesen
dc.subjectChinese medicineen
dc.titleIntervention effects of Ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of Neurotrophin-4 and N-Cadherinen
dc.typeArticleen
dc.contributor.departmentJiamusi Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3634853en

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