A novel zinc finger gene, ZNF465, is inappropriately expressed in presentation acute myeloid leukaemia cells

2.50
Hdl Handle:
http://hdl.handle.net/10547/578755
Title:
A novel zinc finger gene, ZNF465, is inappropriately expressed in presentation acute myeloid leukaemia cells
Authors:
Collin, Joseph F.; Wells, James W.; Czepulkowski, Barbara; Lyne, Linden; Duriez, Patrick J.; Banham, Alison H.; Mufti, Ghulam J.; Guinn, Barbara-Ann
Abstract:
To increase our knowledge of leukaemia-associated antigens, especially in acute myeloid leukaemia (AML) M4, we prepared a phage display cDNA library using mRNA from the bone marrow cells of a patient with AML M4 at diagnosis. We immunoscreened 10(6) pfu with autologous sera and identified an antigen which we named GKT-AML8. The cDNA showed more than 99% similarity to a sequence on 2q21.2 and 95% sequence similarity to a sequence on 19q13.3. These genes were named ZNF465 and ZNF466, respectively, following HUGO Gene Nomenclature Committee (HGNC) guidelines. Expressed sequence tag data suggests that both genes are transcriptionally active. ZNF465 and ZNF466 encode a 5' krüppel associated box domain typical of negative regulators of gene transcription. We have confirmed the translational start site in the +1 frame in a near-Kozak sequence that produces a 102 amino acid polypeptide from ZNF465. The high level of sequence similarity between ZNF465 and ZNF466 makes their transcripts almost indistinguishable by real-time polymerase chain reaction (RT-PCR). However, GKT-AML8 showed most sequence similarity to ZNF465 and no transcript matching the 3' ZNF466 sequence could be detected in patient samples or healthy volunteers. ZNF465/466 expression was detectable in 12/13 AML and 10/14 chronic myeloid leukaemia patients' samples but not in normal donor peripheral blood (0/8) or 0/3 bone marrow samples which had been separated into CD34(+) and CD34(-) samples. The altered expression of ZNF465/466 in patients' samples and its absence in healthy donor haematopoietic samples indicate that ZNF465 is overexpressed in early myeloid disease and as such may represent a promising target for immunotherapy.
Affiliation:
King's College London; University of Oxford; Southampton University Hospitals Trust; University of Bedfordshire
Citation:
Collin, J.F., Wells, J., Czepulkowski, B., Lyne, L., Duriez, P., Banham, A.H., Mufti, G.J. & Guinn, B.A. (2015) 'A novel zinc finger gene, ZNF465, is inappropriately expressed in presentation acute myeloid leukaemia cells.' Genes, Chromosomes & Cancer, Vol. 54, pp. 288-302.
Publisher:
Wiley
Journal:
Genes, Chromosomes & Cancer
Issue Date:
May-2015
URI:
http://hdl.handle.net/10547/578755
DOI:
10.1002/gcc.22242
PubMed ID:
25706801
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1002/gcc.22242/full
Type:
Article
Language:
en
ISSN:
1098-2264
Appears in Collections:
Biomedicine and Nutrition Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorCollin, Joseph F.en
dc.contributor.authorWells, James W.en
dc.contributor.authorCzepulkowski, Barbaraen
dc.contributor.authorLyne, Lindenen
dc.contributor.authorDuriez, Patrick J.en
dc.contributor.authorBanham, Alison H.en
dc.contributor.authorMufti, Ghulam J.en
dc.contributor.authorGuinn, Barbara-Annen
dc.date.accessioned2015-09-25T09:49:03Zen
dc.date.available2015-09-25T09:49:03Zen
dc.date.issued2015-05en
dc.identifier.citationCollin, J.F., Wells, J., Czepulkowski, B., Lyne, L., Duriez, P., Banham, A.H., Mufti, G.J. & Guinn, B.A. (2015) 'A novel zinc finger gene, ZNF465, is inappropriately expressed in presentation acute myeloid leukaemia cells.' Genes, Chromosomes & Cancer, Vol. 54, pp. 288-302.en
dc.identifier.issn1098-2264en
dc.identifier.pmid25706801en
dc.identifier.doi10.1002/gcc.22242en
dc.identifier.urihttp://hdl.handle.net/10547/578755en
dc.description.abstractTo increase our knowledge of leukaemia-associated antigens, especially in acute myeloid leukaemia (AML) M4, we prepared a phage display cDNA library using mRNA from the bone marrow cells of a patient with AML M4 at diagnosis. We immunoscreened 10(6) pfu with autologous sera and identified an antigen which we named GKT-AML8. The cDNA showed more than 99% similarity to a sequence on 2q21.2 and 95% sequence similarity to a sequence on 19q13.3. These genes were named ZNF465 and ZNF466, respectively, following HUGO Gene Nomenclature Committee (HGNC) guidelines. Expressed sequence tag data suggests that both genes are transcriptionally active. ZNF465 and ZNF466 encode a 5' krüppel associated box domain typical of negative regulators of gene transcription. We have confirmed the translational start site in the +1 frame in a near-Kozak sequence that produces a 102 amino acid polypeptide from ZNF465. The high level of sequence similarity between ZNF465 and ZNF466 makes their transcripts almost indistinguishable by real-time polymerase chain reaction (RT-PCR). However, GKT-AML8 showed most sequence similarity to ZNF465 and no transcript matching the 3' ZNF466 sequence could be detected in patient samples or healthy volunteers. ZNF465/466 expression was detectable in 12/13 AML and 10/14 chronic myeloid leukaemia patients' samples but not in normal donor peripheral blood (0/8) or 0/3 bone marrow samples which had been separated into CD34(+) and CD34(-) samples. The altered expression of ZNF465/466 in patients' samples and its absence in healthy donor haematopoietic samples indicate that ZNF465 is overexpressed in early myeloid disease and as such may represent a promising target for immunotherapy.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/gcc.22242/fullen
dc.subjectleukaemiaen
dc.subjectacute myeloid leukaemiaen
dc.subjectZNF465en
dc.titleA novel zinc finger gene, ZNF465, is inappropriately expressed in presentation acute myeloid leukaemia cellsen
dc.typeArticleen
dc.contributor.departmentKing's College Londonen
dc.contributor.departmentUniversity of Oxforden
dc.contributor.departmentSouthampton University Hospitals Trusten
dc.contributor.departmentUniversity of Bedfordshireen
dc.identifier.journalGenes, Chromosomes & Canceren

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