Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes

2.50
Hdl Handle:
http://hdl.handle.net/10547/560930
Title:
Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes
Authors:
Tsai, Sujune; Chen, Jiann-Chu; Spikings, Emma ( 0000-0001-8387-2098 ) ; Li, Jan-Jung; Lin, Chiahsin
Abstract:
A critical step for successful cryopreservation is to determine the optimal cryoprotectant treatment that can provide protective effects against cryoinjury during freezing and with minimal toxicity. Most cryoprotectants have chemical and osmotic effects when used at high concentrations. Cryoprotectants can damage coral mitochondrial distributions and membrane potentials, which results in reduced ATP production. As mitochondrial DNA (mtDNA) encodes for components of the electron transport chain (ETC) and plays a critical role in ATP synthesis capacity, we determined the effects of cryoprotectants on mtDNA in hard coral (Echinopora spp.) oocytes using quantitative real-time PCR. Our results showed that an insult from a cryoprotectant may be compensated for by the genetic defense mechanisms of these cells. Methanol was found to have the least effect on coral oocytes with regard to their energy status. A single oocyte without cryoprotectant treatment produced an average of 4,220,645 ± 169,990 mtDNA copies, which was greater than that in mammals. However, relatively lower mtDNA copy numbers (<2,000,000) were observed when oocytes were treated with dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), or glycerol at a concentration of 3 M for 20 min. These results provide direct evidence that hard coral (Echinopora spp.) oocytes are extremely susceptible to cryoprotectants and support the concerns with regard to the adverse effects of cryoprotectants.
Affiliation:
Mingdao University; National Taiwan Ocean University; University of Bedfordshire; Nationall Museum Marine Biology & Aquarium, Taiwan; National Dong Hwa University
Citation:
Tsai, S.J., Chen, J.C., Spikings, E., Li, J.J. and Lin, C.H. (2015) 'Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes', Mitochondrial DNA, 26(3), pp.420-425.
Publisher:
Informa Healthcare
Journal:
Mitochondrial DNA
Issue Date:
Jun-2015
URI:
http://hdl.handle.net/10547/560930
DOI:
10.3109/19401736.2013.855734
Additional Links:
http://informahealthcare.com/doi/abs/10.3109/19401736.2013.855734
Type:
Article
Language:
en
ISSN:
1940-1736; 1940-1744
Appears in Collections:
Biomedical Science

Full metadata record

DC FieldValue Language
dc.contributor.authorTsai, Sujuneen
dc.contributor.authorChen, Jiann-Chuen
dc.contributor.authorSpikings, Emmaen
dc.contributor.authorLi, Jan-Jungen
dc.contributor.authorLin, Chiahsinen
dc.date.accessioned2015-07-24T09:21:24Zen
dc.date.available2015-07-24T09:21:24Zen
dc.date.issued2015-06en
dc.identifier.citationTsai, S.J., Chen, J.C., Spikings, E., Li, J.J. and Lin, C.H. (2015) 'Degradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytes', Mitochondrial DNA, 26(3), pp.420-425.en
dc.identifier.issn1940-1736en
dc.identifier.issn1940-1744en
dc.identifier.doi10.3109/19401736.2013.855734en
dc.identifier.urihttp://hdl.handle.net/10547/560930en
dc.description.abstractA critical step for successful cryopreservation is to determine the optimal cryoprotectant treatment that can provide protective effects against cryoinjury during freezing and with minimal toxicity. Most cryoprotectants have chemical and osmotic effects when used at high concentrations. Cryoprotectants can damage coral mitochondrial distributions and membrane potentials, which results in reduced ATP production. As mitochondrial DNA (mtDNA) encodes for components of the electron transport chain (ETC) and plays a critical role in ATP synthesis capacity, we determined the effects of cryoprotectants on mtDNA in hard coral (Echinopora spp.) oocytes using quantitative real-time PCR. Our results showed that an insult from a cryoprotectant may be compensated for by the genetic defense mechanisms of these cells. Methanol was found to have the least effect on coral oocytes with regard to their energy status. A single oocyte without cryoprotectant treatment produced an average of 4,220,645 ± 169,990 mtDNA copies, which was greater than that in mammals. However, relatively lower mtDNA copy numbers (<2,000,000) were observed when oocytes were treated with dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), or glycerol at a concentration of 3 M for 20 min. These results provide direct evidence that hard coral (Echinopora spp.) oocytes are extremely susceptible to cryoprotectants and support the concerns with regard to the adverse effects of cryoprotectants.en
dc.language.isoenen
dc.publisherInforma Healthcareen
dc.relation.urlhttp://informahealthcare.com/doi/abs/10.3109/19401736.2013.855734en
dc.titleDegradation of mitochondrial DNA in cryoprotectant-treated hard coral (Echinopora spp.) oocytesen
dc.typeArticleen
dc.contributor.departmentMingdao Universityen
dc.contributor.departmentNational Taiwan Ocean Universityen
dc.contributor.departmentUniversity of Bedfordshireen
dc.contributor.departmentNationall Museum Marine Biology & Aquarium, Taiwanen
dc.contributor.departmentNational Dong Hwa Universityen
dc.identifier.journalMitochondrial DNAen
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