Cryopreservation of zebrafish (Danio rerio) blastomeres using controlled slow cooling

5.00
Hdl Handle:
http://hdl.handle.net/10547/347063
Title:
Cryopreservation of zebrafish (Danio rerio) blastomeres using controlled slow cooling
Authors:
Creamer, Delta Patricia Menendez
Abstract:
Cryopreservation of aquatic species has been widely studied especially zebrafish gametes, embryos and larvae. Cryopreservation of blastomeres has the advantage of preserving both paternal and maternal genetic information. The research work presented in this thesis investigated the toxicity of cryoprotectants to 75% epiboly stage blastomeres of zebrafish (Danio rerio) before cryopreservation. DMSO was found to be the least toxic cryoprotectant for blastomeres, after 30 min incubated in PBS at room temperature, with 97.8% survivaL Cryopreservation of zebrafish blastomeres was carried out using controlled slow cooling. In addition to cryoprotectant, the cryoprotective properties of other compounds were also investigated including NaHC03, coffee and honey, and their solutions were used as cryopreservation media. Comparison of blastomere survival in different media after freezing showed honey to be the most effective, with 98.1 % survival immediately after freeze thawing. Comparison of blastomers cryopreserved in honey and DMSO, after 60 min incubation in PBS at room temperature following freeze-thawing and cryoprotectant removal, showed honey to be the more effective cryoprotectant for controlled slow cooling of zebrafish blastomeres with 97.2% survival. Results from the 'present study showed honey to have properties that protect the blastomere from freezing injury.
Citation:
Creamer, D.P.M. (2008) 'Cryopreservation of zebrafish (Danio rerio) blastomeres using controlled slow cooling'. MSc by Research thesis. University of Bedfordshire.
Publisher:
University of Bedfordshire
Issue Date:
Jul-2008
URI:
http://hdl.handle.net/10547/347063
Type:
Thesis or dissertation
Language:
en
Description:
A thesis submitted for the degree of Master by Research of the University of Bedfordshire.
Appears in Collections:
Masters e-theses

Full metadata record

DC FieldValue Language
dc.contributor.authorCreamer, Delta Patricia Menendezen
dc.date.accessioned2015-03-24T12:28:11Zen
dc.date.available2015-03-24T12:28:11Zen
dc.date.issued2008-07en
dc.identifier.citationCreamer, D.P.M. (2008) 'Cryopreservation of zebrafish (Danio rerio) blastomeres using controlled slow cooling'. MSc by Research thesis. University of Bedfordshire.en
dc.identifier.urihttp://hdl.handle.net/10547/347063en
dc.descriptionA thesis submitted for the degree of Master by Research of the University of Bedfordshire.en
dc.description.abstractCryopreservation of aquatic species has been widely studied especially zebrafish gametes, embryos and larvae. Cryopreservation of blastomeres has the advantage of preserving both paternal and maternal genetic information. The research work presented in this thesis investigated the toxicity of cryoprotectants to 75% epiboly stage blastomeres of zebrafish (Danio rerio) before cryopreservation. DMSO was found to be the least toxic cryoprotectant for blastomeres, after 30 min incubated in PBS at room temperature, with 97.8% survivaL Cryopreservation of zebrafish blastomeres was carried out using controlled slow cooling. In addition to cryoprotectant, the cryoprotective properties of other compounds were also investigated including NaHC03, coffee and honey, and their solutions were used as cryopreservation media. Comparison of blastomere survival in different media after freezing showed honey to be the most effective, with 98.1 % survival immediately after freeze thawing. Comparison of blastomers cryopreserved in honey and DMSO, after 60 min incubation in PBS at room temperature following freeze-thawing and cryoprotectant removal, showed honey to be the more effective cryoprotectant for controlled slow cooling of zebrafish blastomeres with 97.2% survival. Results from the 'present study showed honey to have properties that protect the blastomere from freezing injury.en
dc.language.isoenen
dc.publisherUniversity of Bedfordshireen
dc.subjectC410 Applied Geneticsen
dc.subjectcryopreservationen
dc.subjectzebrafishen
dc.subjectDanio rerioen
dc.subjectcoolingen
dc.titleCryopreservation of zebrafish (Danio rerio) blastomeres using controlled slow coolingen
dc.typeThesis or dissertationen
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