Influence of hypoxic preconditioning in-vivo to 30 minutes knee surgery specific tourniquet application

2.50
Hdl Handle:
http://hdl.handle.net/10547/336249
Title:
Influence of hypoxic preconditioning in-vivo to 30 minutes knee surgery specific tourniquet application
Authors:
Barrington, James Henry
Abstract:
Purpose: To establish whether a bout of hypoxic preconditioning (HPC) or ischemic preconditioning (IPC) would elicit a reduction in total knee replacement (TKR) surgery specific tourniquet mediated oxidative stress (OS) in-vivo. Methods: In an independent group design, 18 healthy men were exposed to 40 min of either: whole-body HPC (14.3% O2), IPC (four bouts of 5 min ischemia and 5 min reperfusion) or rest (SHAM), 1 h prior to 30 min TKR specific limb ischemia and 2 h reperfusion. Systemic blood samples were taken at pre- and post-intervention, additionally blood and gastrocnemius samples were obtained at pre-, 15 min post- (15PoT) and 120 min post-tourniquet deflation. Systemic leukocytes and gastrocnemius tissue were analysed for the heat shock protein (Hsp72) and Heat shock protein 32 (Hsp32) gene transcript response (indicates severity of the cellular stress response), with the systemic plasma also assessed for OS markers (protein carbonyl and glutathione (reduced, oxidised, total, reduced/oxidised-ratio)). Results: A 1.93 and 1.97 fold reduction in gastrocnemius Hsp72 was noted in individuals exposed to HPC (p = 0.007) and IPC (p = 0.006) respectively, in comparison to SHAM at 15PoT. No significant differences were observed in gastrocnemius Hsp32, systemic Hsp72, Hsp32 or OS markers (p > 0.05) between groups. Conclusions: HPC and IPC provided cytoprotection to ischemic stressed gastrocnemius tissue as indicated by an attenuated cellular stress response to 30 min TKR specific limb ischemia.
Citation:
Barrington, J.H. (2013) 'Influence of hypoxic preconditioning in-vivo to 30 minutes knee surgery specific tourniquet application'. MSc by research thesis. University of Bedfordshire.
Publisher:
University of Bedfordshire
Issue Date:
Oct-2013
URI:
http://hdl.handle.net/10547/336249
Type:
Thesis or dissertation
Language:
en
Description:
A thesis submitted to the University of Bedfordshire in partial fulfilment of the requirements for the degree of Masters of Science by Research
Appears in Collections:
Masters e-theses

Full metadata record

DC FieldValue Language
dc.contributor.authorBarrington, James Henryen
dc.date.accessioned2014-11-27T11:12:36Z-
dc.date.available2014-11-27T11:12:36Z-
dc.date.issued2013-10-
dc.identifier.citationBarrington, J.H. (2013) 'Influence of hypoxic preconditioning in-vivo to 30 minutes knee surgery specific tourniquet application'. MSc by research thesis. University of Bedfordshire.en
dc.identifier.urihttp://hdl.handle.net/10547/336249-
dc.descriptionA thesis submitted to the University of Bedfordshire in partial fulfilment of the requirements for the degree of Masters of Science by Researchen
dc.description.abstractPurpose: To establish whether a bout of hypoxic preconditioning (HPC) or ischemic preconditioning (IPC) would elicit a reduction in total knee replacement (TKR) surgery specific tourniquet mediated oxidative stress (OS) in-vivo. Methods: In an independent group design, 18 healthy men were exposed to 40 min of either: whole-body HPC (14.3% O2), IPC (four bouts of 5 min ischemia and 5 min reperfusion) or rest (SHAM), 1 h prior to 30 min TKR specific limb ischemia and 2 h reperfusion. Systemic blood samples were taken at pre- and post-intervention, additionally blood and gastrocnemius samples were obtained at pre-, 15 min post- (15PoT) and 120 min post-tourniquet deflation. Systemic leukocytes and gastrocnemius tissue were analysed for the heat shock protein (Hsp72) and Heat shock protein 32 (Hsp32) gene transcript response (indicates severity of the cellular stress response), with the systemic plasma also assessed for OS markers (protein carbonyl and glutathione (reduced, oxidised, total, reduced/oxidised-ratio)). Results: A 1.93 and 1.97 fold reduction in gastrocnemius Hsp72 was noted in individuals exposed to HPC (p = 0.007) and IPC (p = 0.006) respectively, in comparison to SHAM at 15PoT. No significant differences were observed in gastrocnemius Hsp32, systemic Hsp72, Hsp32 or OS markers (p > 0.05) between groups. Conclusions: HPC and IPC provided cytoprotection to ischemic stressed gastrocnemius tissue as indicated by an attenuated cellular stress response to 30 min TKR specific limb ischemia.en
dc.language.isoenen
dc.publisherUniversity of Bedfordshireen
dc.subjectC600 Sports Scienceen
dc.subjectkneeen
dc.subjectsurgeryen
dc.subjecthypoxic predconditioningen
dc.subjecttourniqueten
dc.titleInfluence of hypoxic preconditioning in-vivo to 30 minutes knee surgery specific tourniquet applicationen
dc.typeThesis or dissertationen
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