Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2

2.50
Hdl Handle:
http://hdl.handle.net/10547/302167
Title:
Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2
Authors:
Eastwood, Daniel C.; Herman, Bram; Noble, Ralph; Dobrovin-Pennington, Andreja; Sreenivasaprasad, Surapareddy; Burton, Kerry S.
Abstract:
Reproductive phase change from vegetative mycelium to the initiation of fruiting in Agaricus bisporus is regulated in large part by the sensing of environmental conditions. A model is proposed in which three separate environmental factors exert control at different stages of the reproductive developmental process change. The eight carbon volatile 1-octen-3-ol controls the early differentiation from vegetative hyphae to multicellular knots; temperature reduction is essential for the later differentiation of primodia; and carbon dioxide level exerts quantitative control on the number of fruiting bodies developed. Analysis of transcriptomic changes during the reproductive phase change was carried out with initiation-specific microarrays, and the newly published A. bisporus genome was used to analyse the promoter regions of differentially regulated genes. Our studies have shown there to be both early and late initiation responses relating to sensing of eight carbon volatiles and temperature respectively. A subset of 45 genes was transcriptionally regulated during the reproductive phase change which exhibited a range of functions including cell structure, nitrogen and carbon metabolism, and sensing and signalling. Three gene clusters linking increased transcription with developmental stage were identified. Analysis of promoter regions revealed cluster-specific conserved motifs indicative of co-ordinated regulation of transcription
Affiliation:
University of Swansea; University of Warwick; East Malling Research; University of Bedfordshire; Agilent Technologies UK Ltd.
Citation:
Eastwood, D.C., Herman, B., Noble, R., Dobrovin-Pennington, A., Sreenivasaprasad, S. and Burton, K.S. (2013) 'Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2', Fungal Genetics and Biology, 55,pp.54-66
Publisher:
Elsevier
Journal:
Fungal Genetics and Biology
Issue Date:
2013
URI:
http://hdl.handle.net/10547/302167
DOI:
10.1016/j.fgb.2013.01.001
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S1087184513000030
Type:
Article
Language:
en
ISSN:
10871845
Appears in Collections:
Environmental Monitoring Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorEastwood, Daniel C.en_GB
dc.contributor.authorHerman, Bramen_GB
dc.contributor.authorNoble, Ralphen_GB
dc.contributor.authorDobrovin-Pennington, Andrejaen_GB
dc.contributor.authorSreenivasaprasad, Surapareddyen_GB
dc.contributor.authorBurton, Kerry S.en_GB
dc.date.accessioned2013-09-24T08:48:41Z-
dc.date.available2013-09-24T08:48:41Z-
dc.date.issued2013-
dc.identifier.citationEastwood, D.C., Herman, B., Noble, R., Dobrovin-Pennington, A., Sreenivasaprasad, S. and Burton, K.S. (2013) 'Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2', Fungal Genetics and Biology, 55,pp.54-66en_GB
dc.identifier.issn10871845-
dc.identifier.doi10.1016/j.fgb.2013.01.001-
dc.identifier.urihttp://hdl.handle.net/10547/302167-
dc.description.abstractReproductive phase change from vegetative mycelium to the initiation of fruiting in Agaricus bisporus is regulated in large part by the sensing of environmental conditions. A model is proposed in which three separate environmental factors exert control at different stages of the reproductive developmental process change. The eight carbon volatile 1-octen-3-ol controls the early differentiation from vegetative hyphae to multicellular knots; temperature reduction is essential for the later differentiation of primodia; and carbon dioxide level exerts quantitative control on the number of fruiting bodies developed. Analysis of transcriptomic changes during the reproductive phase change was carried out with initiation-specific microarrays, and the newly published A. bisporus genome was used to analyse the promoter regions of differentially regulated genes. Our studies have shown there to be both early and late initiation responses relating to sensing of eight carbon volatiles and temperature respectively. A subset of 45 genes was transcriptionally regulated during the reproductive phase change which exhibited a range of functions including cell structure, nitrogen and carbon metabolism, and sensing and signalling. Three gene clusters linking increased transcription with developmental stage were identified. Analysis of promoter regions revealed cluster-specific conserved motifs indicative of co-ordinated regulation of transcriptionen_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1087184513000030en_GB
dc.rightsArchived with thanks to Fungal Genetics and Biologyen_GB
dc.subjectAgaricus bisporusen_GB
dc.subjectreproductive phase changeen_GB
dc.subjectmicroarrayen_GB
dc.subjectfruiting regulationen_GB
dc.subjectcultivated mushroomen_GB
dc.titleEnvironmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2en
dc.typeArticleen
dc.contributor.departmentUniversity of Swanseaen_GB
dc.contributor.departmentUniversity of Warwicken_GB
dc.contributor.departmentEast Malling Researchen_GB
dc.contributor.departmentUniversity of Bedfordshireen_GB
dc.contributor.departmentAgilent Technologies UK Ltd.en_GB
dc.identifier.journalFungal Genetics and Biologyen_GB
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