Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation

2.50
Hdl Handle:
http://hdl.handle.net/10547/302091
Title:
Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation
Authors:
Ahmed, Bushra Y.; Yamaguchi, Fuminori; Tsumura, Toyohiro; Gotoh, Takaya; Sugimoto, Katsuyoshi; Tai, Yuji; Konishi, Ryoji; Kobayashi, Ryoji; Tokuda, Masaaki
Abstract:
Long-term potentiation (LTP) is considered to be associated with an increase in expression as well as activity of Ca2+/calmodulin-dependent protein kinases (CaMKs). LTP-induced and control hippocampal slices were studied by immunohistochemical and electronmicroscopic analyses using anti-CaMK-I, -II and -IV antibodies. All three kinases were demonstrated to increase their expression in CA1 neurons. CaMK-I was shown to mainly localize in the cytoplasm of the control and LTP-induced neurons, and a significant increase of immunoreactivity was observed in the latter neurons. A part of CaMK-I was found to translocate to the nuclei of LTP-induced hippocampal CA1 neurons. Direct evidence of the translocation of CaMK-II from cytoplasm to nuclei in LTP was demonstrated by immuno-electronmicroscopy. A significant increase in expression of CaMK-IV in the nuclei was also observed. Our data suggest that all the three CaMKs were actively involved in nuclear Ca2+- signaling in LTP.
Citation:
Ahmed B. Y., Yamaguchi F., Tsumura T. et al. (2000) 'Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation', Neuroscience Letters, 290(2),pp.149-153
Publisher:
Elsevier
Journal:
Neuroscience Letters
Issue Date:
2000
URI:
http://hdl.handle.net/10547/302091
DOI:
10.1016/S0304-3940(00)01347-1
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0304394000013471
Type:
Article
Language:
en
ISSN:
0304-3940
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorAhmed, Bushra Y.en_GB
dc.contributor.authorYamaguchi, Fuminorien_GB
dc.contributor.authorTsumura, Toyohiroen_GB
dc.contributor.authorGotoh, Takayaen_GB
dc.contributor.authorSugimoto, Katsuyoshien_GB
dc.contributor.authorTai, Yujien_GB
dc.contributor.authorKonishi, Ryojien_GB
dc.contributor.authorKobayashi, Ryojien_GB
dc.contributor.authorTokuda, Masaakien_GB
dc.date.accessioned2013-09-23T11:56:10Z-
dc.date.available2013-09-23T11:56:10Z-
dc.date.issued2000-
dc.identifier.citationAhmed B. Y., Yamaguchi F., Tsumura T. et al. (2000) 'Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation', Neuroscience Letters, 290(2),pp.149-153en_GB
dc.identifier.issn0304-3940-
dc.identifier.doi10.1016/S0304-3940(00)01347-1-
dc.identifier.urihttp://hdl.handle.net/10547/302091-
dc.description.abstractLong-term potentiation (LTP) is considered to be associated with an increase in expression as well as activity of Ca2+/calmodulin-dependent protein kinases (CaMKs). LTP-induced and control hippocampal slices were studied by immunohistochemical and electronmicroscopic analyses using anti-CaMK-I, -II and -IV antibodies. All three kinases were demonstrated to increase their expression in CA1 neurons. CaMK-I was shown to mainly localize in the cytoplasm of the control and LTP-induced neurons, and a significant increase of immunoreactivity was observed in the latter neurons. A part of CaMK-I was found to translocate to the nuclei of LTP-induced hippocampal CA1 neurons. Direct evidence of the translocation of CaMK-II from cytoplasm to nuclei in LTP was demonstrated by immuno-electronmicroscopy. A significant increase in expression of CaMK-IV in the nuclei was also observed. Our data suggest that all the three CaMKs were actively involved in nuclear Ca2+- signaling in LTP.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0304394000013471en_GB
dc.rightsArchived with thanks to Neuroscience Lettersen_GB
dc.subjectlong-term potentiationen_GB
dc.subjectcalmodulin-dependent protein kinaseen_GB
dc.subjecthippocampusen_GB
dc.titleExpression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiationen
dc.typeArticleen
dc.identifier.journalNeuroscience Lettersen_GB
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