Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation
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Authors
Ahmed, Bushra Y.Yamaguchi, Fuminori
Tsumura, Toyohiro
Gotoh, Takaya
Sugimoto, Katsuyoshi
Tai, Yuji
Konishi, Ryoji
Kobayashi, Ryoji
Tokuda, Masaaki
Issue Date
2000
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Show full item recordAbstract
Long-term potentiation (LTP) is considered to be associated with an increase in expression as well as activity of Ca2+/calmodulin-dependent protein kinases (CaMKs). LTP-induced and control hippocampal slices were studied by immunohistochemical and electronmicroscopic analyses using anti-CaMK-I, -II and -IV antibodies. All three kinases were demonstrated to increase their expression in CA1 neurons. CaMK-I was shown to mainly localize in the cytoplasm of the control and LTP-induced neurons, and a significant increase of immunoreactivity was observed in the latter neurons. A part of CaMK-I was found to translocate to the nuclei of LTP-induced hippocampal CA1 neurons. Direct evidence of the translocation of CaMK-II from cytoplasm to nuclei in LTP was demonstrated by immuno-electronmicroscopy. A significant increase in expression of CaMK-IV in the nuclei was also observed. Our data suggest that all the three CaMKs were actively involved in nuclear Ca2+- signaling in LTP.Citation
Ahmed B. Y., Yamaguchi F., Tsumura T. et al. (2000) 'Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation', Neuroscience Letters, 290(2),pp.149-153Publisher
ElsevierJournal
Neuroscience LettersAdditional Links
http://linkinghub.elsevier.com/retrieve/pii/S0304394000013471Type
ArticleLanguage
enISSN
0304-3940ae974a485f413a2113503eed53cd6c53
10.1016/S0304-3940(00)01347-1