Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors

2.50
Hdl Handle:
http://hdl.handle.net/10547/302088
Title:
Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors
Authors:
Ahmed, Bushra Y.; Chakravarthy, Sridhara; Eggers, Ruben; Hermens, Wim TJMC; Zhang, Jing; Niclou, Simone P.; Levelt, Christiaan; Sablitzky, Fred; Anderson, Patrick N.; Lieberman, A.R.; Verhaagen, Joost
Abstract:
Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrain
Affiliation:
University College London; Netherlands Institute for Brain Research; Netherlands Ophthalmic Research Institute; University of Nottingham
Citation:
Ahmed B.Y., Chakravarthy S., Eggers R., et al. (2004) 'Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors', BMC Neuroscience, 5(1):4-13
Publisher:
BioMed Central
Journal:
BMC Neuroscience
Issue Date:
2004
URI:
http://hdl.handle.net/10547/302088
DOI:
10.1186/1471-2202-5-4
Additional Links:
http://www.biomedcentral.com/1471-2202/5/4
Type:
Article
Language:
en
ISSN:
1471-2202
Appears in Collections:
Cell and Cryobiology Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorAhmed, Bushra Y.en_GB
dc.contributor.authorChakravarthy, Sridharaen_GB
dc.contributor.authorEggers, Rubenen_GB
dc.contributor.authorHermens, Wim TJMCen_GB
dc.contributor.authorZhang, Jingen_GB
dc.contributor.authorNiclou, Simone P.en_GB
dc.contributor.authorLevelt, Christiaanen_GB
dc.contributor.authorSablitzky, Freden_GB
dc.contributor.authorAnderson, Patrick N.en_GB
dc.contributor.authorLieberman, A.R.en_GB
dc.contributor.authorVerhaagen, Joosten_GB
dc.date.accessioned2013-09-23T11:39:12Z-
dc.date.available2013-09-23T11:39:12Z-
dc.date.issued2004-
dc.identifier.citationAhmed B.Y., Chakravarthy S., Eggers R., et al. (2004) 'Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors', BMC Neuroscience, 5(1):4-13en_GB
dc.identifier.issn1471-2202-
dc.identifier.doi10.1186/1471-2202-5-4-
dc.identifier.urihttp://hdl.handle.net/10547/302088-
dc.description.abstractInactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the compensatory regulation of other genes. One approach to producing conditional activation or inactivation of genes involves the use of Cre recombinase to remove loxP-flanked segments of DNA. We have studied the effects of delivering Cre to the hippocampus and neocortex of adult mice by injecting replication-deficient adeno-associated virus (AAV) and lentiviral (LV) vectors into discrete regions of the forebrainen_GB
dc.language.isoenen
dc.publisherBioMed Centralen_GB
dc.relation.urlhttp://www.biomedcentral.com/1471-2202/5/4en_GB
dc.rightsArchived with thanks to BMC Neuroscienceen_GB
dc.subjectgeneticsen_GB
dc.subjectnervous systemen_GB
dc.subjectin vivoen_GB
dc.titleEfficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectorsen
dc.typeArticleen
dc.contributor.departmentUniversity College Londonen_GB
dc.contributor.departmentNetherlands Institute for Brain Researchen_GB
dc.contributor.departmentNetherlands Ophthalmic Research Instituteen_GB
dc.contributor.departmentUniversity of Nottinghamen_GB
dc.identifier.journalBMC Neuroscienceen_GB
This item is licensed under a Creative Commons License
Creative Commons
All Items in UOBREP are protected by copyright, with all rights reserved, unless otherwise indicated.