2.50
Hdl Handle:
http://hdl.handle.net/10547/295130
Title:
Daily quadratic trend in basal monocyte expressed HSP72 in healthy human subjects
Authors:
Taylor, Lee; Midgley, Adrian W.; Chrismas, Bryna; Madden, Leigh A; Vince, Rebecca V.; McNaughton, Lars R.
Abstract:
The inducible human stress protein heat shock protein 72 (HSP72) performs vital roles within the body at rest and during periods of stress. Recently it was shown over a 24 hour period that basal HSP72 followed a diurnal variation. However, these results and previous literature demonstrate noticeable inter-subject variation in basal HSP72 expression. The notion of intra/inter-day variation in basal HSP72 expression has not been explored in detail. Basal monocyte expressed HSP72 was determined every 3 hours, over a 9 hour period in 12 healthy male subjects (20.2 +/- 1.9 years, 178.7 +/- 5.6 cm, 75.1 +/- 6.0 kg) within a temperature controlled laboratory. A significant quadratic trend was observed for time (F = 26.0, P = 0.001, partial eta(2) = 0.74), where HSP72 decreased between 0800 and 1100 hours (P < 0.001) and then increased between 1100 and 1400 hours (P = 0.015). The main effect for day (F = 2.6, P = 0.14) and the day x time interaction effect (F = 3.9, P = 0.08) were not significant. There was no correlation between serum and monocyte expressed HSP72, with no significant effect for time (F = 2.0, P = 0.21) in serum HSP72 expression. The results support findings by others that basal monocyte expressed HSP72 follows a diurnal variation which incorporates a quadratic trend, which is not compromised by any significant daily variation and that serum HSP72 expression has no endogenous circadian rhythm. The significant quadratic trend in basal monocyte HSP72 expression shown here highlights the need to tightly control variables, such as timing of sample collection, as it is known basal values influence the magnitude of HSP72 expression post-stressor/intervention.
Affiliation:
Department of Sport, Health and Exercise Science, University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Citation:
Taylor, L., Midgley, A., Madden, L. A., Vince, R. V., Chrismas., B & McNaughton, L. (2010) 'Daily quadratic trend in basal monocyte expressed HSP72 in healthy human subjects', Amino Acids, 38, pp. 1483-1488.
Publisher:
Springer
Journal:
Amino acids
Issue Date:
May-2010
URI:
http://hdl.handle.net/10547/295130
DOI:
10.1007/s00726-009-0360-6
PubMed ID:
19816654
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/19816654
Type:
Article
Language:
en
ISSN:
1438-2199
Appears in Collections:
Applied Sport and Exercise Physiology

Full metadata record

DC FieldValue Language
dc.contributor.authorTaylor, Leeen_GB
dc.contributor.authorMidgley, Adrian W.en_GB
dc.contributor.authorChrismas, Brynaen_GB
dc.contributor.authorMadden, Leigh Aen_GB
dc.contributor.authorVince, Rebecca V.en_GB
dc.contributor.authorMcNaughton, Lars R.en_GB
dc.date.accessioned2013-07-03T08:36:40Z-
dc.date.available2013-07-03T08:36:40Z-
dc.date.issued2010-05-
dc.identifier.citationTaylor, L., Midgley, A., Madden, L. A., Vince, R. V., Chrismas., B & McNaughton, L. (2010) 'Daily quadratic trend in basal monocyte expressed HSP72 in healthy human subjects', Amino Acids, 38, pp. 1483-1488.en_GB
dc.identifier.issn1438-2199-
dc.identifier.pmid19816654-
dc.identifier.doi10.1007/s00726-009-0360-6-
dc.identifier.urihttp://hdl.handle.net/10547/295130-
dc.description.abstractThe inducible human stress protein heat shock protein 72 (HSP72) performs vital roles within the body at rest and during periods of stress. Recently it was shown over a 24 hour period that basal HSP72 followed a diurnal variation. However, these results and previous literature demonstrate noticeable inter-subject variation in basal HSP72 expression. The notion of intra/inter-day variation in basal HSP72 expression has not been explored in detail. Basal monocyte expressed HSP72 was determined every 3 hours, over a 9 hour period in 12 healthy male subjects (20.2 +/- 1.9 years, 178.7 +/- 5.6 cm, 75.1 +/- 6.0 kg) within a temperature controlled laboratory. A significant quadratic trend was observed for time (F = 26.0, P = 0.001, partial eta(2) = 0.74), where HSP72 decreased between 0800 and 1100 hours (P < 0.001) and then increased between 1100 and 1400 hours (P = 0.015). The main effect for day (F = 2.6, P = 0.14) and the day x time interaction effect (F = 3.9, P = 0.08) were not significant. There was no correlation between serum and monocyte expressed HSP72, with no significant effect for time (F = 2.0, P = 0.21) in serum HSP72 expression. The results support findings by others that basal monocyte expressed HSP72 follows a diurnal variation which incorporates a quadratic trend, which is not compromised by any significant daily variation and that serum HSP72 expression has no endogenous circadian rhythm. The significant quadratic trend in basal monocyte HSP72 expression shown here highlights the need to tightly control variables, such as timing of sample collection, as it is known basal values influence the magnitude of HSP72 expression post-stressor/intervention.en_GB
dc.language.isoenen
dc.publisherSpringeren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/19816654en_GB
dc.rightsArchived with thanks to Amino acidsen_GB
dc.subjectC600 Sports Scienceen_GB
dc.subject.meshCircadian Rhythm-
dc.subject.meshHSP72 Heat-Shock Proteins-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMonocytes-
dc.subject.meshReference Values-
dc.titleDaily quadratic trend in basal monocyte expressed HSP72 in healthy human subjectsen
dc.typeArticleen
dc.contributor.departmentDepartment of Sport, Health and Exercise Science, University of Hull, Cottingham Road, Hull, HU6 7RX, UK.en_GB
dc.identifier.journalAmino acidsen_GB

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